Evidence map›Paper›PMID 37902899›Full record

ArticleMolecular diversity2024

WGS-based screening of the co-chaperone protein DjlA-induced curved DNA binding protein A (CbpA) from a new multidrug-resistant zoonotic mastitis-causing Klebsiella pneumoniae strain: a novel molecular target of selective flavonoids.

Mohammad Habibur Rahman, Salauddin Al Azad, Mohammad Fahim Uddin, Maisha Farzana, Iffat Ara Sharmeen, Kaifi Sultana Kabbo, Anika Jabin, Ashfaque Rahman, Farhan Jamil, Sanjida Ahmed Srishti and 7 more

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular diversity, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 10 institutions in 3 countries.

Mohammad Habibur RahmanMolecular Microbiology and Vaccinology Lab, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Salauddin Al AzadKey Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu, People's Republic of China.
Mohammad Fahim UddinCollege of Material Science and Engineering, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, People's Republic of China.
Maisha FarzanaSchool of Medicine, Dentistry and Nursing, University of Glasgow, University Avenue, Glasgow, G12 8QQ, UK.
Iffat Ara SharmeenDepartment of Mathematics & Natural Sciences, School of Data Sciences, BRAC University, Dhaka, 1212, Bangladesh.
Kaifi Sultana KabboDepartment of Biochemistry and Microbiology, North South University, Dhaka, 1229, Bangladesh.
Anika JabinDepartment of Biochemistry and Microbiology, North South University, Dhaka, 1229, Bangladesh.
Ashfaque RahmanDepartment of Biochemistry and Microbiology, North South University, Dhaka, 1229, Bangladesh.
Farhan JamilDepartment of Pharmacy, University of Asia Pacific, Farmgate, Dhaka, 1205, Bangladesh.
Sanjida Ahmed SrishtiSchool of Pharmacy, BRAC University, 66 Mohakhali, Dhaka, 1212, Bangladesh.
Fahmida Haque RiyaSchool of Pharmacy, BRAC University, 66 Mohakhali, Dhaka, 1212, Bangladesh.
Towhid KhanDepartment of Medicine, Comilla Medical College, Kuchaitoli, Comilla, 3500, Bangladesh.
Rasel AhmedSchool of Computing, Engineering and Digital Technologies, Teesside University, Middlesbrough, TS1 3BX, UK.
NurunnaharDepartment of Mathematics, Mawlana Bhashani Science and Technology University, Tangail, 1902, Bangladesh.
Samiur RahmanDepartment of Biochemistry and Microbiology, North South University, Dhaka, 1229, Bangladesh.
Mohammad Ferdousur Rahman KhanMolecular Microbiology and Vaccinology Lab, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Md Bahanur RahmanMolecular Microbiology and Vaccinology Lab, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh. bahanurr@bau.edu.bd.ORCID http://orcid.org/0000-0002-8602-2344
North South University · BDBangladesh Agricultural University · BDBRAC University · BDComilla Medical College · BDJiangnan University · CNMawlana Bhashani Science and Technology University · BDTeesside University · GBUniversity of Asia Pacific · BDUniversity of Glasgow · GBZhejiang Sci-Tech University · CN

Funding

Bangladesh Academy of Science (BAS) and the United States Department of Agriculture (USDA) Endowment Program Grant ID: BAS-USDA LS-26/2020 (4th phase)RPG Interface Lab (Registration No. 05-060-06021) Grant ID: Category-E4-GRP-2021/22 (Phase-2)
6 · The paper itself

Abstract

The research aimed to establish a multidrug-resistant Klebsiella pneumoniae-induced genetic model for mastitis considering the alternative mechanisms of the DjlA-mediated CbpA protein regulation. The Whole Genome Sequencing of the newly isolated K. pneumoniae strain was conducted to annotate the frequently occurring antibiotic resistance and virulence factors following PCR and MALDI-TOF mass-spectrophotometry. Co-chaperon DjlA was identified and extracted via restriction digestion on PAGE. Based on the molecular string property analysis of different DnaJ and DnaK type genes, CbpA was identified to be regulated most by the DjlA protein during mastitis. Based on the quantum tunnel-cluster profiles, CbpA was modeled as a novel target for diversified biosynthetic, and chemosynthetic compounds. Pharmacokinetic and pharmacodynamic analyses were conducted to determine the maximal point-specificity of selective flavonoids in complexing with the CbpA macromolecule at molecular docking. The molecular dynamic simulation (100 ns) of each of the flavonoid-protein complexes was studied regarding the parameters RMSD, RMSF, Rg, SASA, MMGBSA, and intramolecular hydrogen bonds; where all of them resulted significantly. To ratify all the molecular dynamic simulation outputs, the potential stability of the flavonoids in complexing with CbpA can be remarked as Quercetin > Biochanin A > Kaempherol > Myricetin, which were all significant in comparison to the control Galangin. Finally, a comprehensive drug-gene interaction pathway for each of the flavonoids was developed to determine the simultaneous and quantitative-synergistic effects of different operons belonging to the DnaJ-type proteins on the metabolism of the tested pharmacophores in CbpA. Considering all the in vitro and in silico parameters, DjlA-mediated CbpA can be a novel target for the tested flavonoids as the potential therapeutics of mastitis as futuristic drugs.

Indexed as

Bacterial ProteinsDrug Resistance, Multiple, BacterialFlavonoidsKlebsiella pneumoniaeMastitisMolecular Docking SimulationMolecular Dynamics SimulationAnimalsAnti-Bacterial AgentsDNA-Binding ProteinsFemaleHumansKlebsiella InfectionsMolecular ChaperonesWhole Genome SequencingAnti-Bacterial AgentsBacterial ProteinsDNA-Binding ProteinsFlavonoidsMolecular ChaperonesCo-chaperone DjlA proteinDnaK Co-chaperone CbpA proteinMultidrug-resistant K. pneumoniaePharmacokinetics and pharmacodynamics of natural flavonoidsWhole genome sequencing

Identifiers

PMID37902899
OpenAlexW4388022968

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.