ArticleNature microbiology2023
A high-throughput and low-waste viability assay for microbes.
Article in Nature microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
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- Antibacterial surface engineering: bioinspiration from leaves to medical devices.RSC applied interfaces · 2026Review
- Evaluation of an AI-Assisted Colony Counting System Across Multiple Culture Media Using Standardized Pure Culture Plates.Microorganisms · 2026Article
- Bioinspired and living multiscale composites for regenerative medicine in the treatment of surgical site infections.Journal of nanobiotechnology · 2026Review
- Fluorescent carbon dot-based biosensor for the rapid and sensitive detection of Escherichia coli DNA.Scientific reports · 2026Article
- High-Resolution Colony Images of Clinically Isolated Bacteria for Automated Detection and Deep Learning.Scientific data · 2026Article
- Bnip3lb-driven mitophagy maintains fate of the embryonic hematopoietic stem cell pool.Nature communications · 2026Article
- Assessing the cytotoxicity and apoptosis-inducing ability of solar irradiated Salmonella Typhimurium in the RAW264.7 cell line in vitro.Scientific reports · 2026Article
- A two-step screen identifies a small molecule that disrupts membrane voltage and is effective against growing and persister gram-negative bacteria.Microbiology spectrum · 2026Article
- AI-Assisted Impedance Biosensing of Yeast Cell Concentration.Biosensors · 2025Article
- Synthesis and Evaluation of Trehalose-Based Mertansine Warheads for Bacillus Calmette-Guérin Delivery of Anticancer Agents.Chembiochem : a European journal of chemical biology · 2025Article
- Type VI secretion system activity at lethal antibiotic concentrations leads to overestimation of weapon potency.Microbiology (Reading, England) · 2025Article
- A digital plating platform for robust and versatile microbial detection and analysis.Scientific reports · 2025Article
- A high-throughput anaerobic method for viability assays.Microbiology spectrum · 2025Article
- Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II).Scientific reports · 2025Article
- Bio-based polylactic acid labware as a sustainable alternative for microbial cultivation in life science laboratories.Heliyon · 2024Article
- Drug combinations targeting antibiotic resistance.npj antimicrobials and resistance · 2024Review
- A Simple High-Throughput Technology for Microorganism Detection and Quantitative Analysis.Foods (Basel, Switzerland) · 2024Article
- Bacterial killing and the dimensions of bacterial death.NPJ biofilms and microbiomes · 2024Article
- Inhalable tobramycin EEG powder formulation for treating Pseudomonas aeruginosa-induced lung infection.International journal of pharmaceutics · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Counting viable cells is a universal practice in microbiology. The colony-forming unit (CFU) assay has remained the gold standard to measure viability across disciplines, but it is time-intensive and resource-consuming. Here we describe the geometric viability assay (GVA) that replicates CFU measurements over 6 orders of magnitude while reducing over 10-fold the time and consumables required. GVA computes a sample's viable cell count on the basis of the distribution of embedded colonies growing inside a pipette tip. GVA is compatible with Gram-positive and Gram-negative planktonic bacteria (Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis), biofilms and fungi (Saccharomyces cerevisiae). Laborious CFU experiments such as checkerboard assays, treatment time-courses and drug screens against slow-growing cells are simplified by GVA. The ease and low cost of GVA evinces that it can replace existing viability assays and enable viability measurements at previously impractical scales.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.