Evidence map›Paper›PMID 37922456›Full record

ArticleNano letters2023

Ultrasensitive, Multiplexed Buoyant Sensor for Monitoring Cytokines in Biofluids.

Heng Guo, Rohit Gupta, Dhavan Sharma, Elizabeth Zhanov, Connor Malone, Ravi Jada, Ying Liu, Mayank Garg, Srikanth Singamaneni, Feng Zhao and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nano letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Optical Bionanosensors for Sepsis Diagnostics.Small (Weinheim an der Bergstrasse, Germany) · 2025
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Heng GuoDepartment of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.
Rohit GuptaDepartment of Mechanical Engineering and Materials Science, Institute of Materials Science and Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States.
Dhavan SharmaDepartment of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.
Elizabeth ZhanovDepartment of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.
Connor MaloneDepartment of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.
Ravi JadaDepartment of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.
Ying LiuDepartment of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.
Mayank GargDepartment of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.
Srikanth SingamaneniDepartment of Mechanical Engineering and Materials Science, Institute of Materials Science and Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States.ORCID 0000-0002-7203-2613
Feng ZhaoDepartment of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.
Limei TianDepartment of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.ORCID 0000-0002-1931-8567

Funding

Continuous metabolite and protein profiling for immune monitoringR35GM147568 · NIGMS · TEXAS ENGINEERING EXPERIMENT STATION · PI Limei Tian · 2022 to 2026
$1.8M
Refreshable Biosensors for Continuous Renal Function MonitoringR21EB029064 · NIBIB · TEXAS ENGINEERING EXPERIMENT STATION · PI TIAN, LIMEI · 2020 to 2020
$589k
NIBIB NIH HHS R21 EB029064NIGMS NIH HHS R35 GM147568
6 · The paper itself

Abstract

Multiplexed quantification of low-abundance protein biomarkers in complex biofluids is important for biomedical research and clinical diagnostics. However, in situ sampling without perturbing biological systems remains challenging. In this work, we report a buoyant biosensor that enables in situ monitoring of protein analytes at attomolar concentrations with a 15 min temporal resolution. The buoyant biosensor implemented with fluorescent nanolabels enabled the ultrasensitive and multiplexed detection and quantification of cytokines. Implementing the biosensor in a digital manner (i.e., counting the individual nanolabels) further improves the low detection limit. We demonstrate that the biosensor enables the detection and quantification of the time-varying concentrations of cytokines (e.g., IL-6 and TNF-α) in macrophage culture media without perturbing the live cells. The easy-to-apply biosensor with attomolar sensitivity and multiplexing capability can enable an in situ analysis of protein biomarkers in various biofluids and tissues to aid in understanding biological processes and diagnosing and treating diverse diseases.

Indexed as

Biosensing TechniquesCytokinesBiomarkersBiomarkersCytokinescytokinesdigitalimmune biomarkersmultiplexed biosensorsultrasensitive protein detection

Identifiers

PMID37922456
PMCPMC10863391

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.