Evidence map›Paper›PMID 37952497›Full record

ArticleBiophysical chemistry2024

Pomegranate peel, chokeberry leaves and Ironwort extract as novel natural inhibitors of amylin aggregation and cellular toxicity in pancreatic β cells.

Achanta Rishisree, Brayer Mallory, Karnaukhova Elena, Jankovic Teodora, Zdunić Gordana, Šavikin Katarina, Jeremic Aleksandar

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Achanta RishisreeDepartment of Biological Sciences, The George Washington University, Washington, DC 20052, USA.
Brayer MalloryDepartment of Biological Sciences, The George Washington University, Washington, DC 20052, USA.
Karnaukhova ElenaCenter for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD 20993, USA.
Jankovic TeodoraInstitute for Medicinal Plant Research "Dr. Josif Pančić", 11000 Belgrade, Serbia.
Zdunić GordanaInstitute for Medicinal Plant Research "Dr. Josif Pančić", 11000 Belgrade, Serbia.
Šavikin KatarinaInstitute for Medicinal Plant Research "Dr. Josif Pančić", 11000 Belgrade, Serbia.
Jeremic AleksandarDepartment of Biological Sciences, The George Washington University, Washington, DC 20052, USA. Electronic address: jerema@gwu.edu.
George Washington University · USInstitute for Plant Protection and Environment · RSCenter for Biologics Evaluation and Research · US

Funding

Therapeutic ultrasound for stimulation of insulin releaseR01EB027648 · NIBIB · GEORGE WASHINGTON UNIVERSITY · PI ZDERIC, VESNA · 2020 to 2023
$2.0M
Molecular Mechanisims of Amylin Trafficking and Toxicity in Human Pancreatic IsleR01DK091845 · NIDDK · GEORGE WASHINGTON UNIVERSITY · PI JEREMIC, ALEKSANDAR MILORAD · 2013 to 2017
$1.4M
NIBIB NIH HHS R01 EB027648NIDDK NIH HHS R01 DK091845
6 · The paper itself

Abstract

Impeding or reducing human amylin aggregation and/or its toxicity can be key to preventing pancreatic islet amyloidosis and β-cell loss in patients with Type 2 Diabetes Mellitus (T2DM). Here, Punica granatum (pomegranate) peel, Sideritis raeseri (ironwort) and Aronia melanocarpa (chokeberry) leaf extracts, were tested for their novel anti-aggregative and antitoxic properties in human amylin (hIAPP) treated rat pancreatic insulinoma (INS) cells. The protein aggregation (Th-T) assay revealed an inhibitory trend of all three plant extracts against amylin aggregates. In agreement with this finding, pomegranate peel and ironwort extracts effectively prevented the transition of hIAPP from disordered, random coil structures into aggregation prone β-sheet enriched molecular assemblies, revealed by CD spectroscopy. Consistent with their anti-aggregative action, all three extracts prevented, to various degrees, reactive oxygen species (ROS) accumulation, mitochondrial stress, and, ultimately, apoptosis of INS cells. Collectively, the results from this study demonstrate effectiveness of natural products to halt hIAPP aggregation, redox stress, and toxicity, which could be exploited as novel therapeutics against amylin-derived islet amyloidosis and β-cell stress in T2DM.

Indexed as

AmyloidosisDiabetes Mellitus, Type 2Insulin-Secreting CellsPomegranateSideritisAnimalsHumansIslet Amyloid PolypeptidePlant ExtractsRatsIslet Amyloid PolypeptidePlant ExtractsAmylinMitochondrial stressPlant extractsProtein aggregationProteotoxicityReactive oxygen species

Identifiers

PMID37952497
PMCPMC10841580
OpenAlexW4388089428

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.