Evidence map›Paper›PMID 37961092›Full record

ArticlebioRxiv : the preprint server for biology2023

The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer.

Mohamed I Khalil, Canchai Yang, Lexi Vu, Smriti Chadha, Harrison Nabors, Claire D James, Iain M Morgan, Dohun Pyeon

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Mohamed I KhalilDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA.
Canchai YangDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA.
Lexi VuDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA.
Smriti ChadhaDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA.
Harrison NaborsDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA.
Claire D JamesPhilips Institute for Oral Health Research, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia, USA.
Iain M MorganPhilips Institute for Oral Health Research, School of Dentistry, Virginia Commonwealth University, Richmond, Virginia, USA.ORCID 0000-0002-4949-3032
Dohun PyeonDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA.ORCID 0000-0003-1970-844X
Michigan State University · USVirginia Commonwealth University · USNational Research Centre · EG

Funding

Chemokine-mediated antigen-specific T cell responses and immunotherapies to treat head and neck cancerR01DE029524 · NIDCR · MICHIGAN STATE UNIVERSITY · PI PYEON, DOHUN, SPANOS, WILLIAM CHARLES · 2020 to 2024
$3.2M
Immune Dysregulation by Human Papillomavirus during Head and Neck Cancer ProgressionR01DE026125 · NIDCR · UNIVERSITY OF COLORADO DENVER · PI PYEON, DOHUN, SPANOS, WILLIAM CHARLES · 2016 to 2020
$2.8M
NIDCR NIH HHS R01 DE026125NIDCR NIH HHS R01 DE029524
6 · The paper itself

Abstract

The human papillomavirus (HPV) oncoprotein E7 is a relatively short-lived protein required for HPV-driven cancer development and maintenance. E7 is degraded through ubiquitination mediated by cullin 1 (CUL1) and the ubiquitin-conjugating enzyme E2 L3 (UBE2L3). However, E7 proteins are maintained at high levels in most HPV-positive cancer cells. A previous proteomics study has shown that UBE2L3 and CUL1 protein levels are increased by the knockdown of the E3 ubiquitin ligase membrane-associated ring-CH-type finger 8 (MARCHF8). We have recently demonstrated that HPV upregulates MARCHF8 expression in HPV-positive keratinocytes and head and neck cancer (HPV+ HNC) cells. Here, we report that MARCHF8 stabilizes the E7 protein by degrading the components of the SKP1-CUL1-F-box (SCF) ubiquitin ligase complex in HPV+ HNC cells. We found that

Identifiers

PMID37961092
PMCPMC10635129
OpenAlexW4388385236

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.