Evidence map›Paper›PMID 37993476›Full record

ArticleScientific reports2023

Fluorescent molecular rotors as versatile in situ sensors for protein quantitation.

Kevin Daus, Sorachat Tharamak, Wanchai Pluempanupat, Peter A Galie, Maria A Theodoraki, Emmanuel A Theodorakis, Mary L Alpaugh

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kevin Daus *Department of Biological and Biomedical Sciences, Rowan University, 201 Mullica Hill Rd, Glassboro, NJ, 08028, USA.
Sorachat Tharamak *Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0358, USA.
Wanchai PluempanupatDepartment of Chemistry and Center of Excellence for Innovation in Chemistry, Special Research Unit for Advanced Magnetic Resonance, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Peter A GalieDepartment of Biomedical Engineering, Rowan University, Glassboro, NJ, 08028, USA.
Maria A TheodorakiDepartment of Biology, Arcadia University, 450 S. Easton Rd, Glenside, PA, 19038, USA.ORCID 0000-0002-0775-8928
Emmanuel A TheodorakisDepartment of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0358, USA. etheodorakis@ucsd.edu.ORCID 0000-0001-9845-6919
Mary L AlpaughDepartment of Biological and Biomedical Sciences, Rowan University, 201 Mullica Hill Rd, Glassboro, NJ, 08028, USA. alpaugh@rowan.edu.ORCID 0000-0002-0008-2718

Funding

Chemical design of amyloid targeting fluorescent probesRF1AG062362 · NIA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI THEODORAKIS, EMMANUEL A, YANG, JERRY · 2018 to 2020
$2.1M
NIA NIH HHS RF1 AG062362
6 · The paper itself

Abstract

Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of assay-specific reagents and importantly, is a terminal analysis. This study exploits the unique chemical features of a fluorescent molecular rotor that fluctuates between twisted-to-untwisted states, with a subsequent intensity increase in fluorescence depending on environmental conditions (e.g., viscosity). Here we report the development of a rapid, sensitive in situ protein quantitation method using ARCAM-1, a representative fluorescent molecular rotor that can be employed in both non-terminal and terminal assays.

Indexed as

Fluorescent DyesFluorescenceViscosityFluorescent Dyes

Identifiers

PMID37993476
PMCPMC10665405

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.