Evidence map›Paper›PMID 38019338›Full record

ArticleBiochemical genetics2024

circ0005027 Acting as a ceRNA Affects the Malignant Biological Behavior of Hypopharyngeal Squamous Cell Carcinoma by Modulating miR-548c-3p/CDH1 Axis.

Xi Yang, Chun Feng, Donghui Jiang, Xin Xu, Yingying Zhang, Jin Wang, Xiaoguang He

Open access · hybridAbstract read
PubMed Publisher
In one paragraph

Article in Biochemical genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Xi YangThe Second Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Rd, Kunming, 650032, Yunnan, China.
Chun FengDepartment of Otolaryngology, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, 650034, Yunnan, China.
Donghui JiangThe Second Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Rd, Kunming, 650032, Yunnan, China.
Xin XuThe Second Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Rd, Kunming, 650032, Yunnan, China.
Yingying ZhangDepartment of Pathology and Pathophysiology, School of Basic Medical Science, Kunming Medical University, Kunming, 650500, Yunnan, China.
Jin WangDepartment of Otolaryngology, The Second People's Hospital of Yunnan Province, Kunming, 650021, Yunnan, China.
Xiaoguang HeThe Second Department of Otolaryngology Head and Neck Surgery, First Affiliated Hospital of Kunming Medical University, No. 295 Xichang Rd, Kunming, 650032, Yunnan, China. hexg1018@163.com.
First Affiliated Hospital of Kunming Medical University · CNKunming Medical University · CNKunming University of Science and Technology · CNSecond People's Hospital of Yunnan Province · CN

Funding

Yunnan Provincial Department of Science and Technology-Kunming Medical University Joint Project on Applied Basic Research 2019FE001(-007)
6 · The paper itself

Abstract

Hypopharyngeal squamous cell carcinoma (HSCC) is a malignant tumor of head and neck. It was verified that circ0005027 was downregulated in HSCC tissues. Here, we aimed to investigate the function and specific regulatory mechanism of circ0005027 in HSCC. Ten pairs tissues of HSCC and adjacent para-cancer were collected. Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) measured circ0005027, miR-548c-3p, and Cadherin 1 (CDH1) mRNA expression. CCK-8 analyzed cell proliferation viability. Flow cytometry assay detected cell cycle and apoptosis rate. Clonal formation assay measured the clonal ability. Transwell detected cell invasion ability. Western blot was performed to detect CDH1, LAST1, p-LAST1, MST1, p-MST1, YAP1, p-YAP1, TAZ and p-TAZ protein level. Dual-luciferase, RIP and RNA pull-down assay identified the target relationship among circ0005027, miR-548c-3p and CDH1. circ0005027 was decreased in tissues and FaDu cells of HSCC. Overexpression of circ0005027 inhibited cell viability, G1-S transition, clonal formation, and invasion and increased cell apoptosis. circ0005027 acted as a ceRNA and decreased circ0005027 enhanced the malignant process of FaDu cells through sponging miR-548c-3p and inhibiting CDH1 expression. Overexpression of CDH1 activated YAP1/TAZ pathway and inhibited the growth of HSCC in vitro. circ0005027 might act as a potential biomarker for the progression and prognosis prediction in HSCC by regulating miR-548c-3p/CDH1/ YAP1/TAZ signaling pathway.

Indexed as

Antigens, CDCadherinsHypopharyngeal NeoplasmsMicroRNAsApoptosisCarcinoma, Squamous CellCell Line, TumorCell ProliferationFemaleGene Expression Regulation, NeoplasticHumansRNA, Competitive EndogenousSquamous Cell Carcinoma of Head and NeckAntigens, CDCadherinsCDH1 protein, humanMicroRNAsMIRN548 microRNA, humanRNA, Competitive EndogenousApoptosiscirc0005027Hypopharyngeal squamous cell carcinomaMalignant processmiR-548c-3pProliferation

Identifiers

PMID38019338
OpenAlexW4389138836

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.