Evidence map›Paper›PMID 38039367›Full record

ArticleScience advances2023

Genetically engineered transfusable platelets using mRNA lipid nanoparticles.

Jerry Leung, Colton Strong, Katherine E Badior, Madelaine Robertson, Xiaowu Wu, Michael A Meledeo, Emma Kang, Manoj Paul, Yusuke Sato, Hideyoshi Harashima and 5 more

Open access · goldAbstract read
In one paragraph

Article in Science advances, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
7.0field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Review
  3. Engineering platelets as cancer therapeutics.Nature reviews. Clinical oncology · 2026
    Review
  4. Intravenous infusion of engineered megakaryocytes to produce oncolytic platelets in vivo for enhanced cancer immunotherapy.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  5. Article
  6. Article
  7. Materials today. Bio · 2025
    Article
  8. Oncogenic KRASInternational journal of molecular sciences · 2025
    Article
  9. Review
  10. Article
  11. Review
  12. Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 3 countries.

Jerry LeungMichael Smith Laboratories, University of British Columbia, Vancouver, V6T 1Z4, Canada.ORCID 0000-0002-8714-8440
Colton StrongMichael Smith Laboratories, University of British Columbia, Vancouver, V6T 1Z4, Canada.ORCID 0000-0003-4031-9932
Katherine E BadiorBlood Research Institute, Versiti, Milwaukee,WI 53226, USA.ORCID 0000-0003-1215-0500
Madelaine RobertsonMichael Smith Laboratories, University of British Columbia, Vancouver, V6T 1Z4, Canada.ORCID 0000-0002-1764-0245
Xiaowu WuBlood and Shock Resuscitation Program, United States Army Institute of Surgical Research, JBSA-FT Sam Houston, San Antonio, TX 78234, USA.ORCID 0000-0002-1024-0900
Michael A MeledeoBlood and Shock Resuscitation Program, United States Army Institute of Surgical Research, JBSA-FT Sam Houston, San Antonio, TX 78234, USA.ORCID 0000-0001-9958-9115
Emma KangCentre for Blood Research, University of British Columbia, Vancouver, V6T 1Z3, Canada.
Manoj PaulBlood Research Institute, Versiti, Milwaukee,WI 53226, USA.
Yusuke SatoLaboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Hokkaido, 060-0812, Japan.ORCID 0000-0003-0913-7815
Hideyoshi HarashimaLaboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Hokkaido, 060-0812, Japan.
Andrew P CapBlood and Shock Resuscitation Program, United States Army Institute of Surgical Research, JBSA-FT Sam Houston, San Antonio, TX 78234, USA.ORCID 0000-0003-0357-0597
Dana V DevineDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, V6T 1Z3, Canada.
Eric JanDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, V6T 1Z3, Canada.ORCID 0000-0003-0523-8467
Pieter R CullisDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, V6T 1Z3, Canada.
Christian J KastrupMichael Smith Laboratories, University of British Columbia, Vancouver, V6T 1Z4, Canada.ORCID 0000-0003-3644-0019
University of British Columbia · CAUnited States Army Institute of Surgical Research · USHokkaido Pharmaceutical University · JPVersiti Blood Center of Wisconsin · US

Funding

Frederick Banting and Charles Best Doctoral Award 6552U.S. Department of Defense Investigator-Initiated Research Award W81XWH202004
6 · The paper itself

Abstract

Platelet transfusions are essential for managing bleeding and hemostatic dysfunction and could be expanded as a cell therapy due to the multifunctional role of platelets in various diseases. Creating these cell therapies will require modifying transfusable donor platelets to express therapeutic proteins. However, there are currently no appropriate methods for genetically modifying platelets collected from blood donors. Here, we describe an approach using platelet-optimized lipid nanoparticles containing mRNA (mRNA-LNP) to enable exogenous protein expression in human and rat platelets. Within the library of mRNA-LNP tested, exogenous protein expression did not require nor correlate with platelet activation. Transfected platelets retained hemostatic function and accumulated in regions of vascular damage after transfusion into rats with hemorrhagic shock. We expect this technology will expand the therapeutic potential of platelets.

Indexed as

Blood PlateletsHemostaticsAnimalsBlood DonorsHumansLiposomesNanoparticlesRatsRNA, MessengerHemostaticsLipid NanoparticlesLiposomesRNA, Messenger

Identifiers

PMID38039367
PMCPMC10691771
OpenAlexW4389233268

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.