ArticleFrontiers in veterinary science2023
Storage of boar semen at 17°C without conventional antibiotics in an extender containing an organic bactericidal substance.
Article in Frontiers in veterinary science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Storage-associated shifts in the raw boar seminal microbiome at 17 °C.Frontiers in veterinary science · 2026Article
- Ferroptosis contributes to boar sperm deterioration during liquid semen storage.Frontiers in veterinary science · 2026Article
- Pre-cooling of boar semen before transport in a hot environment enhances biosafety.Frontiers in microbiology · 2025Article
- Article
- Fertility after photodynamic inactivation of bacteria in extended boar semen.Frontiers in microbiology · 2024Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Facing the global threat of antimicrobial resistance, the reduction of antibiotic use in semen extenders is a main goal in artificial insemination (AI) of pigs. The aim of this study was to investigate the potential of a commercial extender containing an organic bactericidal supplement in the absence of conventional antibiotics to control bacterial growth and to maintain the quality of boar spermatozoa during long-term semen storage for up to 144 h at 17°C. Methods: Semen from 233 boars housed at 16 European AI centers was split and diluted in the long-term extender "Androstar Plus without antibiotics + organic bactericidal supplement" (APlus) and in the control extender Beltsville Thawing Solution (BTS) with gentamicin, which is routinely used in many AI centers. Sperm motility was assessed with computer-assisted semen analysis (CASA) and membrane integrity was evaluated with flow cytometry. The number of bacteria was determined by counting colonies on agar plates. Results: At the end of storage, bacterial counts were ≥ 10 Conclusion: Long-term storage of boar semen at 17°C without conventional antibiotics in an extender containing an organic bactericidal supplement is an option if semen samples are regularly tested for the presence of
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Registered trials
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