Evidence map›Paper›PMID 38076839›Full record

ArticlebioRxiv : the preprint server for biology2023

Perineuronal nets and the neuronal extracellular matrix can be imaged by genetically encoded labeling of HAPLN1

Sakina P Lemieux, Varda Lev-Ram, Roger Y Tsien, Mark H Ellisman

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Sakina P LemieuxDepartment of Neurosciences, University of California, San Diego, La Jolla CA 92093-0647.ORCID 0000-0001-8143-5839
Varda Lev-RamDepartment of Neurosciences, University of California, San Diego, La Jolla CA 92093-0647.
Roger Y TsienDepartment of Neurosciences, University of California, San Diego, La Jolla CA 92093-0647.
Mark H EllismanDepartment of Neurosciences, University of California, San Diego, La Jolla CA 92093-0647.ORCID 0000-0001-8893-8455
University of California San Diego · USHoward Hughes Medical Institute · US

Funding

IVEM and Image Analysis ResourceP41GM103412 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2012 to 2019
$17.9M
GRADUATE TRAINING IN CELLULAR &MOLECULAR PHARMACOLOGYT32GM007752 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BROWN, JOAN HELLER, HANDEL, TRACY M · 1985 to 2023
$13.5M
TRAINING IN THE NEUROPLASTICITY OF AGINGT32AG000216 · NIA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KOO, EDWARD H. · 1992 to 2017
$8.4M
New Probe and Methods for Correlated LM & EMR01GM086197 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ADAMS, STEPHEN ROY, BOASSA, DANIELA · 2008 to 2020
$8.3M
DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONSR01NS027177 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ADAMS, STEPHEN ROY, ELLISMAN, MARK H · 1989 to 2017
$5.8M
National Center for Microscopy and Imaging Research: A BRAIN Technology Integration and Dissemination ResourceU24NS120055 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELLISMAN, MARK H · 2021 to 2025
$5.4M
DYNAMICS OF SIGNAL TRANSDUCTION IN NEURONSR37NS027177 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TSIEN, ROGER Y · 2007 to 2013
$4.8M
NIA NIH HHS T32 AG000216NIGMS NIH HHS P41 GM103412NIGMS NIH HHS R01 GM086197NIGMS NIH HHS T32 GM007752NINDS NIH HHS R01 NS027177NINDS NIH HHS R37 NS027177NINDS NIH HHS U24 NS120055
6 · The paper itself

Abstract

Neuronal extracellular matrix (ECM) and a specific form of ECM called the perineuronal net (PNN) are important structures for central nervous system (CNS) integrity and synaptic plasticity. PNNs are distinctive, dense extracellular structures that surround parvalbumin (PV)-positive inhibitory interneurons with openings at mature synapses. Enzyme-mediated PNN disruption can erase established memories and re-open critical periods in animals, suggesting that PNNs are important for memory stabilization and conservation. Here, we characterized the structure and distribution of several ECM/PNN molecules around neurons in culture, brain slice, and whole mouse brain. While specific lectins are well-established as PNN markers and label a distinct, fenestrated structure around PV neurons, we show that other CNS neurons possess similar extracellular structures assembled around hyaluronic acid, suggesting a PNN-like structure of different composition that is more widespread. We additionally report that genetically encoded labeling of hyaluronan and proteoglycan link protein 1 (HAPLN1) reveals a PNN-like structure around many neurons

Identifiers

PMID38076839
PMCPMC10705503
OpenAlexW4389209722

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.