Evidence map›Paper›PMID 38085372›Full record

ArticleMolecular biology reports2023

Reduced Proteolipid Protein 2 promotes endoplasmic reticulum stress-related apoptosis and increases drug sensitivity in acute myeloid leukemia.

Fahui Xie, Jia Qu, Dainan Lin, Kexin Feng, Mingzhu Tan, Haixiu Liao, Longhui Zeng, Qingquan Xiong, Jun Huang, Weiwen Chen

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Fahui XieDepartment of Clinical Laboratory, Guangzhou Twelfth People's Hospital, Guangzhou Medical University, Guangzhou, China.
Jia QuDepartment of Hematology, Guangzhou Twelfth People's Hospital, Guangzhou Medical University, Guangzhou, China.
Dainan LinDepartment of Hematology, Guangzhou Twelfth People's Hospital, Guangzhou Medical University, Guangzhou, China.
Kexin FengDepartment of Hematology, Guangzhou Twelfth People's Hospital, Guangzhou Medical University, Guangzhou, China.
Mingzhu TanDepartment of Hematology, Guangzhou Twelfth People's Hospital, Guangzhou Medical University, Guangzhou, China.
Haixiu LiaoDepartment of Clinical Laboratory, Guangzhou Twelfth People's Hospital, Guangzhou Medical University, Guangzhou, China.
Longhui ZengDepartment of Organ Transplantation, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Qingquan XiongDepartment of Basic Medical Science, Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou, China.
Jun HuangDepartment of Basic Medical Science, Sino-French Hoffmann Institute, Guangzhou Medical University, Guangzhou, China. hj165@sina.com.
Weiwen ChenDepartment of Clinical Laboratory, Guangzhou Twelfth People's Hospital, Guangzhou Medical University, Guangzhou, China. chenweiwen85144052@163.com.
Guangzhou Medical University · CNTwelfth Guangzhou City People's Hospital · CN

Funding

The City-School (Hospital) Joint Project of Guangzhou Basic Research Program SL2024A03J00766The Guangzhou Twelfth People's Hospital Program 2022-1-01The Science and Technology Program of Guangzhou 202002030022
6 · The paper itself

Abstract

backgroundThe Proteolipid Protein 2 (PLP2), a protein in the Endoplasmic Reticulum (ER) membrane, has been reported to be highly expressed in various tumors. Previous studies have demonstrated that the reduced PLP2 can induce apoptosis and autophagy through ER stress-related pathways, leading to a decreased proliferation and aggressiveness. However, there is no research literature on the role of PLP2 in Acute Myeloid Leukemia (AML).

methodsPLP2 expression, clinical data, genetic mutations, and karyotype changes from GEO, TCGA, and timer2.0 databases were analyzed through the R packages. The possible functions and pathways of cells were explored through GO, KEGG, and GSEA enrichment analysis using the clusterProfiler R package. Immuno-infiltration analysis was conducted using the Cibersort algorithm and the Xcell R package. RT-PCR and western blot techniques were employed to identify the PLP2 expression, examine the knockdown effects in THP-1 cells, and assess the expression of genes associated with endoplasmic reticulum stress and apoptosis. Flow cytometry was utilized to determine the apoptosis and survival rates of different groups.

resultsPLP2 expression was observed in different subsets of AML and other cancers. Enrichment analyses revealed that PLP2 was involved in various tumor-related biological processes, primarily apoptosis and lysosomal functions. Additionally, PLP2 expression showed a strong association with immune cell infiltration, particularly monocytes. In vitro, the knockdown of PLP2 enhanced endoplasmic reticulum stress-related apoptosis and increased drug sensitivity in THP-1 cells.

conclusionsPLP2 could be a novel therapeutic target in AML, in addition, PLP2 is a potential endoplasmic reticulum stress regulatory gene in AML.

Indexed as

ApoptosisLeukemia, Myeloid, AcuteEndoplasmic ReticulumEndoplasmic Reticulum StressHumansMARVEL Domain-Containing ProteinsProteolipidsMARVEL Domain-Containing ProteinsPLP2 protein, humanProteolipidsAcute myeloid leukemiaApoptosisEndoplasmic reticulum stressThe Proteolipid Protein 2Treatment

Identifiers

PMID38085372
OpenAlexW4389613744

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.