Evidence map›Paper›PMID 38106159›Full record

ArticlebioRxiv : the preprint server for biology2023

Fibroblast Growth Factor-induced lens fiber cell elongation is driven by the stepwise activity of Rho and Rac.

Yuki Sugiyama, Daniel A Reed, David Herrmann, Frank J Lovicu, Michael L Robinson, Paul Timpson, Ichiro Masai

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 3 countries.

Yuki SugiyamaDevelopmental Neurobiology Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.ORCID 0000-0001-7830-0677
Daniel A ReedCancer Ecosystems Program, The Garvan Institute of Medical Research & The Kinghorn Cancer Centre, St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia.
David HerrmannCancer Ecosystems Program, The Garvan Institute of Medical Research & The Kinghorn Cancer Centre, St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia.
Frank J LovicuSave Sight Institute, The University of Sydney, Sydney, NSW 2000, Australia.
Michael L RobinsonDepartment of Biology and Center for Visual Sciences, Miami University, Oxford, OH, USA.
Paul TimpsonCancer Ecosystems Program, The Garvan Institute of Medical Research & The Kinghorn Cancer Centre, St Vincent's Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia.
Ichiro MasaiDevelopmental Neurobiology Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan.ORCID 0000-0002-6626-6595
Garvan Institute of Medical Research · AUThe University of Sydney · AUMiami University · USOkinawa Institute of Science and Technology Graduate University · JP

Funding

STUDIES ON LENS DIFFERENTIATION: ROLE OF FGFR01EY003177 · NEI · UNIVERSITY OF SYDNEY · PI LOVICU, FRANK JAMES, MCAVOY, JOHNSTON W · 1985 to 2015
$1.8M
NEI NIH HHS R01 EY003177
6 · The paper itself

Abstract

The spheroidal shape of the eye lens is critical for precise light focusing onto the retina. This shape is determined by concentrically aligned, convexly elongated lens fiber cells along the anterior and posterior axis of the lens. Upon differentiation at the lens equator, the fiber cells increase in height as their apical and basal tips migrate towards the anterior and posterior poles, respectively. The forces driving this elongation and migration remain unclear. We found that membrane protrusions or lamellipodia are observed only in the maturing fibers undergoing cell curve conversion, indicating lamellipodium is not the primary driver of earlier fiber migration. We demonstrated that elevated levels of fibroblast growth factor (FGF) suppressed the extension of Rac-dependent protrusions, suggesting changes in the activity of FGF controling Rac activity, switching to lamellipodium-driven migration. Inhibitors of ROCK, myosin, and actin reduced the height of both early and later fibers, indicating elongation of these fibers relies on actomyosin contractility. Consistently, active RhoA was detected throughout these fibers. Given that FGF promotes fiber elongation, we propose it to do so through regulation of Rho activity.

Indexed as

cell shapeFGFLensRacRhotissue morphogenesis

Identifiers

PMID38106159
PMCPMC10723307
OpenAlexW4389333442

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.