Evidence map›Paper›PMID 38110485›Full record

ArticleScientific reports2023

LncRNA SNHG8 regulates the migration and angiogenesis of pHUVECs induced by high glucose via the TRPM7/ERK

Zongcheng Fan, Xin Chen, Laicheng Wang, Jianjian Yu, Shunpeng Zhang, Changsheng Xu, Jinxiu Lin, Yunchai Lin, Feng Peng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Zongcheng Fan *The First Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, China.
Xin Chen *Department of Cardiology, National Regional Medical Center of Binhai Campus of the First Affiliated Hospital of Fujian Medical University, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
Laicheng Wang *The First Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, China.
Jianjian YuThe First Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, China.
Shunpeng ZhangThe First Clinical Medical College, Fujian Medical University, Fuzhou, Fujian, China.
Changsheng XuFujian Provincial Institute of Hypertension, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
Jinxiu LinDepartment of Cardiology, National Regional Medical Center of Binhai Campus of the First Affiliated Hospital of Fujian Medical University, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
Yunchai LinDepartment of Cardiology, National Regional Medical Center of Binhai Campus of the First Affiliated Hospital of Fujian Medical University, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. yunchailin@163.com.
Feng PengDepartment of Cardiology, National Regional Medical Center of Binhai Campus of the First Affiliated Hospital of Fujian Medical University, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. pengfeng@fjmu.edu.cn.
Fujian Medical University · CNFirst Affiliated Hospital of Fujian Medical University · CN

Funding

Foreign Cooperation Project of Science and Technology, Fujian Province 2021I0013Fujian provincial health technology project 2020QNA056Joint Funds for the innovation of science and Technology，Fujian province 2021Y9121Joint Funds for the Innovation of Science and Technology,Fujian Province 2021Y9144National Natural Science Foundation of China 81970370Natural Science Fundation of Fujian Province 2022J01686Scientific Research Project of Hefei Third People's Hospital SYKZ202201The scientific research personnel training project for the Fujian Provincial Health Commission 2019-1-40
6 · The paper itself

Abstract

This study aimed to evaluate the regulatory effect and molecular mechanism of long noncoding RNA small nucleolus RNA host gene 8 (LncRNA SNHG8) in the migration and angiogenesis of primary human umbilical vein endothelial cells (pHUVECs) under high-glucose (HG) conditions. The HG-induced endothelial injury model was established in vitro.The cell model of silencing SNHG8, overexpressing SNHG8, and silencing TRPM7 was established by transfecting SNHG8-siRNA, SNHG8 plasmid and TRPM7-siRNA into cells with liposomes.The SNHG8 level was determined through reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression levels of transient receptor potential melastatin 7 (TRPM7), endothelial nitric oxide synthase (eNOS), p-eNOS, extracellular signal-regulated kinase 1/2(ERK1/2), and p-ERK1/2 were assessed through western blot. Nitric oxide (NO) levels were measured with DAF-FM. pHUVEC migration was examined through wound healing and Transwell assay, and pHUVEC angiogenesis was observed through a tube formation assay. Results showed that HG promoted the expression of lncRNA SNHG8 and TRPM7 and decreased the ratio of p-eNOS/eNOS and p-ERK

Indexed as

RNA, Long NoncodingTRPM Cation ChannelsAngiogenesisButadienesGlucoseHumansHuman Umbilical Vein Endothelial CellsMitogen-Activated Protein Kinase 3NitrilesProtein Serine-Threonine KinasesRNA, Small InterferingButadienesGlucoseMitogen-Activated Protein Kinase 3NitrilesProtein Serine-Threonine KinasesRNA, Long NoncodingRNA, Small InterferingTRPM7 protein, humanTRPM Cation ChannelsU 0126

Identifiers

PMID38110485
PMCPMC10728107
OpenAlexW4389895301

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.