Evidence map›Paper›PMID 38140666›Full record

ArticleViruses2023

Involvement of a Rarely Used Splicing SD2b Site in the Regulation of HIV-1

Takaaki Koma, Naoya Doi, Bao Quoc Le, Tomoyuki Kondo, Mitsuki Ishizue, Chiaki Tokaji, Chizuko Tsukada, Akio Adachi, Masako Nomaguchi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Takaaki KomaDepartment of Microbiology, Graduate School of Medicine, Tokushima University, Tokushima 770-8503, Japan.ORCID 0000-0001-9767-990X
Naoya DoiDepartment of Microbiology, Graduate School of Medicine, Tokushima University, Tokushima 770-8503, Japan.
Bao Quoc LeDepartment of Microbiology, Graduate School of Medicine, Tokushima University, Tokushima 770-8503, Japan.
Tomoyuki KondoDepartment of Microbiology, Graduate School of Medicine, Tokushima University, Tokushima 770-8503, Japan.
Mitsuki IshizueDepartment of Microbiology, Graduate School of Medicine, Tokushima University, Tokushima 770-8503, Japan.
Chiaki TokajiDepartment of Microbiology, Graduate School of Medicine, Tokushima University, Tokushima 770-8503, Japan.
Chizuko TsukadaDepartment of Microbiology, Graduate School of Medicine, Tokushima University, Tokushima 770-8503, Japan.
Akio AdachiDepartment of Microbiology, Graduate School of Medicine, Tokushima University, Tokushima 770-8503, Japan.ORCID 0000-0003-1674-332X
Masako NomaguchiDepartment of Microbiology, Graduate School of Medicine, Tokushima University, Tokushima 770-8503, Japan.

Funding

AMED 21443907AMED 22580694JSPS 21K0704200JSPS 21K0849100JSPS 22K0710200JSPS 23K0794000
6 · The paper itself

Abstract

We have previously reported an HIV-1 mutant designated NL-Y226tac that expresses Vif at an ultra-low level, being replication-defective in high-APOBEC3G cells, such as H9. It carries a synonymous mutation within the splicing SA1 site relative to its parental clone. In order to determine whether a certain mutant(s) emerges during multi-infection cycles, we maintained H9 cells infected with a relatively low or high input of NL-Y226tac for extended time periods. Unexpectedly, we reproducibly identified a g5061a mutation in the SD2b site in the two independent long-term culture experiments that partially increases Vif expression and replication ability. Importantly, the adaptive mutation g5061a was demonstrated to enhance

Indexed as

HIV-1Cell LineCytidine DeaminaseMutationRNA, Messengervif Gene Products, Human Immunodeficiency VirusVirus ReplicationCytidine DeaminaseRNA, Messengervif Gene Products, Human Immunodeficiency VirusadaptationAPOBEC3HIV-1SA1SD2bsplicingVif

Identifiers

PMID38140666
PMCPMC10747208

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.