Evidence map›Paper›PMID 38155285›Full record

ArticleMolecular biotechnology2025

Identification and Validation of Functional miRNAs and Their Main Targets in Sorghum bicolor.

Abdul Baqi, Samiullah, Wajid Rehman, Iram Bibi, Farid Menaa, Yousaf Khan, Doha A Albalawi, Abdul Sattar

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Article in Molecular biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 3 countries.

Abdul BaqiDepartment of Chemistry, University of Balochistan, Quetta, 87300, Pakistan.ORCID http://orcid.org/0000-0001-9978-6362
SamiullahDepartment of Chemistry, University of Balochistan, Quetta, 87300, Pakistan.ORCID http://orcid.org/0000-0003-2193-4542
Wajid RehmanDepartment of Chemistry, Hazara University Mansehra, Mansehra, Pakistan. sono_waj@yahoo.com.ORCID http://orcid.org/0000-0003-0128-0377
Iram BibiDepartment of Chemistry, Hazara University Mansehra, Mansehra, Pakistan.ORCID http://orcid.org/0000-0002-5304-6235
Farid MenaaDepartment of Biomedical and Environmental Engineering (BEE), California Innovations Corporation (CIC), San Diego, CA, 92037, USA. menaateam@gmail.com.ORCID http://orcid.org/0000-0002-0258-7322
Yousaf KhanDepartment of Chemistry, COMSATS University Islamabad, Islamabad, 45550, Pakistan.ORCID http://orcid.org/0000-0001-5455-7849
Doha A AlbalawiDepartment of Biology, Faculty of Science, University of Tabuk, 71491, Tabuk, Saudi Arabia.
Abdul SattarDepartment of Chemistry, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
COMSATS University Islamabad · PKHazara University · PKUniversity of Balochistan · PKCalifornia Medical Innovations Institute · USUniversity of Tabuk · SA

Funding

Hazara University HU/699/2021
6 · The paper itself

Abstract

MicroRNAs (miRNAs) are typically non-coding RNAs of 18-26 nucleotides (nts) that are produced endogenously and regulated post-transcriptionally through degradation or translational repression. Since miRNAs are evolutionarily conserved, their preservation is essential for important regulatory functions in plant development, growth, and responses to environmental stress. Sorghum bicolor (sbi) is a valuable food and fodder crop which is grown worldwide. A range of sbi miRNAs were identified so far as being connected to plant development and stress responses. Herein, we employed a variety of bioinformatics tools for miRNA profiling in sbi and a PCR-based platform for the validation of these miRNAs. In total, 74 new conserved sbi miRNAs from 52 miRNA families have been predicted. Using the psRNA Target method, 10613 different protein targets of these predicted miRNAs have been attained. These targets include 54 GO-terms which have substantial targets in the biological, molecular, and cellular processes. We particularly found that the sbi-miR1861c and sbi-miR5050 are involved to regulate sulphur compound biosynthetic process, while the significant spliceosomal complex is regulated by sbi-miR815b and sbi-miR7768b. Also, we report that the pre-ribosome, electron transport chain, cell communication, cellular respiration, protein localization, and photosynthesis are controlled by sbi-miR2907b, sbi-miR530, sbi-miR7749, sbi-miR1858a, sbi-mi7729a, and sbi-miR417, respectively. The identification and validation of these novel sbi miRNAs shall contribute a lot in improving the crop yield and ensure sustainable agriculture.

Indexed as

Gene Expression Regulation, PlantMicroRNAsRNA, PlantSorghumComputational BiologyGene Expression ProfilingPlant ProteinsMicroRNAsPlant ProteinsRNA, PlantmiRNAMolecular functionProtein targetsRT-PCRSorghum bicolorSustainability

Identifiers

PMID38155285
OpenAlexW4390325892

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.