Evidence map›Paper›PMID 38159259›Full record

ArticleAging2023

ANGPTL4, a direct target of hsa-miR-133a-3p, accelerates lung adenocarcinoma lipid metabolism, proliferation and invasion.

Qihao Hu, Shi Chen, Yukun Li, Teng Hu, Jianpeng Hu, Cheng Wang, Fei Yang, Xiang Yang, Feng Zhou, Zhengdong Liu and 2 more

Open access · hybridAbstract read
In one paragraph

Article in Aging, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.8field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Qihao HuDepartment of Thoracic Surgery, The First People’s Hospital of Changde City, Changde, Hunan, China.
Shi ChenDepartment of Thoracic Surgery, The First People’s Hospital of Changde City, Changde, Hunan, China.
Yukun LiDepartment of Assisted Reproductive Centre, Zhuzhou Central Hospital, Xiangya Hospital Zhuzhou Central South University, Central South University, Zhuzhou, Hunan, China.
Teng HuDepartment of Pathology, The First People’s Hospital of Changde City, Changde, Hunan, China.
Jianpeng HuDepartment of Thoracic Surgery, The First People’s Hospital of Changde City, Changde, Hunan, China.
Cheng WangDepartment of Thoracic Surgery, The First People’s Hospital of Changde City, Changde, Hunan, China.
Fei YangDepartment of Thoracic Surgery, The First People’s Hospital of Changde City, Changde, Hunan, China.
Xiang YangDepartment of Thoracic Surgery, The First People’s Hospital of Changde City, Changde, Hunan, China.
Feng ZhouDepartment of Thoracic Surgery, The First People’s Hospital of Changde City, Changde, Hunan, China.
Zhengdong LiuDepartment of Thoracic Surgery, The First People’s Hospital of Changde City, Changde, Hunan, China.
Wei XuDepartment of Thoracic Surgery, The First People’s Hospital of Changde City, Changde, Hunan, China.
Ji ZhangDepartment of Thoracic Surgery, The First People’s Hospital of Changde City, Changde, Hunan, China.
The First People's Hospital of Changde · CNZhuzhou Central Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGlobally, lung adenocarcinoma (LUAD) is the most common type of lung cancer. The secreted protein angiopoietin-like 4 (ANGPTL4) has been implicated in a number of physiological and pathological processes, including angiogenesis and lipid metabolism. But the role of ANGPTL4 in LUAD remains unknown.

methodsThe expression of ANGPTL4 and miR-133a-3p was confirmed by public database analysis. Xenograft model, MTT, Clone formation and EdU analysis were used to confirm the effects of miR-133a-3p/ANGPTL4 on LUAD cell proliferation and growth. Wound healing and Transwell analysis were used to elucidate the role of miR-133a-3p/ANGPTL4 in LUAD cell migration and invasion. Oil red O staining was used to confirm ANGPTL4 in LUAD lipids production. Dual-luciferase reporter gene analysis was used to demonstrate miR-133a-3p could directly bind ANGPTL4 3'-UTR. WB and PCR were used to confirm the protein expression of ANGPTL4.

resultsANGPTL4 was significantly increased in LUAD samples, which could promote LUAD cell proliferation, migration, invasion, growth and lipid production. miR-133a-3p could directly bind to ANGPTL4 mRNA, and repress the expression ANGPTL4, resulting in suppressing LUAD proliferation and metastasis.

conclusionIn conclusion, miR-133a-3p/ANGPTL4 axis might be a potential biomarker and therapeutic target for LUAD patients.

Indexed as

Adenocarcinoma of LungAngiopoietin-Like Protein 4Cell MovementCell ProliferationLipid MetabolismLung NeoplasmsMicroRNAsNeoplasm InvasivenessAnimalsCell Line, TumorGene Expression Regulation, NeoplasticHumansMiceMice, NudeAngiopoietin-Like Protein 4ANGPTL4 protein, humanMicroRNAsMIRN133 microRNA, humanANGPTL4bioinformaticshsa-miR-133a-3plipid metabolism reprogramminglung adenocarcinoma

Identifiers

PMID38159259
PMCPMC11132016
OpenAlexW4390404273

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.