BAF45D-binding to HOX genes was differentially targeted in H9-derived spinal cord neural stem cells.

Chang Liu, Yuxin Xie, Xueying Chen, Lihua Liu, Chao Liu, Zongsheng Yin

Abstract read

In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

6 authors.

Chang LiuDepartment of Orthopedics, The First Affiliated Hospital, Anhui Medical University, Hefei, 230032, Anhui, China.

Yuxin XieDepartment of Histology and Embryology, Institute of Stem Cell and Tissue Engineering, School of Basic Medical Sciences, Anhui Medical University, Hefei, 230032, Anhui, China.

Xueying ChenDepartment of Histology and Embryology, Institute of Stem Cell and Tissue Engineering, School of Basic Medical Sciences, Anhui Medical University, Hefei, 230032, Anhui, China.

Lihua LiuInstitute of Clinical Pharmacology, Anhui Medical University, Hefei, 230032, Anhui, China.

Chao LiuDepartment of Histology and Embryology, Institute of Stem Cell and Tissue Engineering, School of Basic Medical Sciences, Anhui Medical University, Hefei, 230032, Anhui, China. chaol1974@ahmu.edu.cn.

Zongsheng YinDepartment of Orthopedics, The First Affiliated Hospital, Anhui Medical University, Hefei, 230032, Anhui, China. yinzongsheng@sina.com.

Funding

Key Research and Development Project of Anhui Province 202004J07020037Natural Science Foundation of Anhui Province 2008085MH251Research project of Anhui Provincial Institute of Translational Medicine 2021zhyx-C19

6 · The paper itself

Abstract

Chromatin accessibility has been used to define how cells adopt region-specific neural fates. BAF45D is one of the subunits of a specialised chromatin remodelling BAF complex. It has been reported that BAF45D is expressed in spinal cord neural stem cells (NSCs) and regulates their fate specification. Within the developing vertebrate spinal cord, HOX genes exhibit spatially restricted expression patterns. However, the chromatin accessibility of BAF45D binding HOX genes in spinal cord NSCs is unclear. In the present study, we found that in H9-derived spinal cord NSCs, BAF45D targets TBX6, a gene that regulates spinal cord neural mesodermal progenitors. Furthermore, BAF45D binding to the NES gene is much more enriched in H9-derived spinal cord NSCs chromatin compared to ESCs chromatin. In addition, BAF45D binding to anterior and trunk/central HOX genes, but not to lumbosacral HOX genes, was much more enriched in NSCs chromatin compared to ESCs chromatin. These results may shed new light on the role of BAF45D in regulating region-specific spinal cord NSCs by targeting HOX genes.

Indexed as

Neural Stem CellsSpinal Cord InjuriesChromatinGenes, HomeoboxHumansSpinal CordT-Box Domain ProteinsTranscription FactorsChromatinT-Box Domain ProteinsTBX6 protein, humanTranscription Factors

Identifiers

PMID38168763

DOI10.1038/s41598-023-50939-y

PMCPMC10761701

What Socratic holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.