Evidence map›Paper›PMID 38172513›Full record

ArticleScientific reports2024

New dienelactone hydrolase from microalgae bacterial community-Antibiofilm activity against fish pathogens and potential applications for aquaculture.

Lutgardis Bergmann, Simone Balzer Le, Gunhild Hageskal, Lena Preuss, Yuchen Han, Yekaterina Astafyeva, Simon Loevenich, Sarah Emmann, Pablo Perez-Garcia, Daniela Indenbirken and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Lutgardis BergmannDepartment of Microbiology and Biotechnology, Institute of Plant Science and Microbiology, University of Hamburg, Ohnhorststr.18, 22609, Hamburg, Germany.
Simone Balzer LeDepartment of Biotechnology and Nanomedicine, SINTEF Industry, Trondheim, Norway.
Gunhild HageskalDepartment of Biotechnology and Nanomedicine, SINTEF Industry, Trondheim, Norway.
Lena PreussDepartment of Microbiology and Biotechnology, Institute of Plant Science and Microbiology, University of Hamburg, Ohnhorststr.18, 22609, Hamburg, Germany.
Yuchen HanDepartment of Microbiology and Biotechnology, Institute of Plant Science and Microbiology, University of Hamburg, Ohnhorststr.18, 22609, Hamburg, Germany.
Yekaterina AstafyevaDepartment of Microbiology and Biotechnology, Institute of Plant Science and Microbiology, University of Hamburg, Ohnhorststr.18, 22609, Hamburg, Germany.
Simon LoevenichDepartment of Biotechnology and Nanomedicine, SINTEF Industry, Trondheim, Norway.
Sarah EmmannMolecular Microbiology, Institute for General Microbiology, Kiel University, Kiel, Germany.
Pablo Perez-GarciaMolecular Microbiology, Institute for General Microbiology, Kiel University, Kiel, Germany.
Daniela IndenbirkenVirus Genomics, Leibniz Institute of Virology, Hamburg, Germany.
Elena KatzowitschCore Unit Systems Medicine, University of Würzburg, Würzburg, Germany.
Fritz ThümmlerCore Unit Systems Medicine, University of Würzburg, Würzburg, Germany.
Malik AlawiBioinformatics Core, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Alexander WentzelDepartment of Biotechnology and Nanomedicine, SINTEF Industry, Trondheim, Norway.
Wolfgang R StreitDepartment of Microbiology and Biotechnology, Institute of Plant Science and Microbiology, University of Hamburg, Ohnhorststr.18, 22609, Hamburg, Germany.
Ines KrohnDepartment of Microbiology and Biotechnology, Institute of Plant Science and Microbiology, University of Hamburg, Ohnhorststr.18, 22609, Hamburg, Germany. ines.krohn@uni-hamburg.de.

Funding

Bundesministerium für Bildung und Forschung BaPro (FKZ 031B0846G)Bundesministerium für Bildung und Forschung MarbioTech (FKZ 161B0562A, RCN 281742)ERA-Net BlueBio Cofund AquaHealth (FKZ 161B0945C, RCN 312075)ERA-Net BlueBio Cofund SureMetS (FKZ 161B0944A)
6 · The paper itself

Abstract

Biofilms are resistant to many traditional antibiotics, which has led to search for new antimicrobials from different and unique sources. To harness the potential of aquatic microbial resources, we analyzed the meta-omics datasets of microalgae-bacteria communities and mined them for potential antimicrobial and quorum quenching enzymes. One of the most interesting candidates (Dlh3), a dienelactone hydrolase, is a α/β-protein with predicted eight α-helices and eight β-sheets. When it was applied to one of the major fish pathogens, Edwardsiella anguillarum, the biofilm development was reproducibly inhibited by up to 54.5%. The transcriptome dataset in presence of Dlh3 showed an upregulation in functions related to self-defense like active genes for export mechanisms and transport systems. The most interesting point regarding the biotechnological potential for aquaculture applications of Dlh3 are clear evidence of biofilm inhibition and that health and division of a relevant fish cell model (CHSE-214) was not impaired by the enzyme.

Indexed as

Anti-Infective AgentsMicroalgaeAnimalsAnti-Bacterial AgentsAquacultureBacteriaBiofilmsCarboxylic Ester HydrolasesFishesQuorum SensingAnti-Bacterial AgentsAnti-Infective AgentsCarboxylic Ester Hydrolasescarboxymethylenebutenolidase

Identifiers

PMID38172513
PMCPMC10764354

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.