Evidence map›Paper›PMID 38189396›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2023

[Characterization of a 3-dimensional tuberculosis spheroid model constructed using human monocytic THP-1 cells and

J Guo, Y Qiu, C Hu, D Li, Y DU

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

J GuoSchool of Biomedical Engineering, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Y QiuSchool of Biomedical Engineering, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
C HuSchool of Biomedical Engineering, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
D LiDepartment of Respiratory and Critical Care Medicine, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Y DUSchool of Biomedical Engineering, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
The Affiliated Yongchuan Hospital of Chongqing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo establish a 3-dimensional tuberculosis spheroid model for studying the formation and characteristics of tuberculous granuloma

methodsHuman myeloid leukemia mononuclear THP-1 cells and

resultsIn the cell-bacterial suspensions, stable 3-D tuberculous spheroids (50-200 μm) occurred slowly, in which the cells adhered tightly with numerous bacteria in the center, and necrotic cells and monocytederived macrophages were seen within the spheroids. Drug penetration was difficult in the 3D tuberculous spheroids as compared with the non-infected cell spheroids. Transmission electron microscopy revealed the presence of cell necrosis and a large number of BCG in the macrophages in the tuberculous spheroids. The tuberculosis spheroid had a more hypoxic microenvironment than the non-infected cell spheroids with higher H

conclusionThe 3-D tuberculous spheroid model established in this study provides a useful platform for studies of tuberculous granuloma.

Indexed as

Mycobacterium bovisTuberculosisAnti-Bacterial AgentsBCG VaccineGranulomaHumansHydrogen PeroxideHypoxiaMonocytesRifampinTHP-1 CellsAnti-Bacterial AgentsBCG VaccineHydrogen PeroxideRifampindrug penetrationmicroenvironmenttuberculosis spheroid modeltuberculous granuloma

Identifiers

PMID38189396
PMCPMC10774115
OpenAlexW4390743939

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.