Evidence map›Paper›PMID 38189953›Full record

ArticleApplied microbiology and biotechnology2024

Biosynthesis of eriodictyol in citrus waster by endowing P450BM3 activity of naringenin hydroxylation.

Xingyi Zhang, Yinghui Feng, Yuanzhe Hua, Chuanxi Zhang, Bohuan Fang, Xiang Long, Yue Pan, Bei Gao, John Z H Zhang, Lijun Li and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Xingyi Zhang *College of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Yinghui Feng *Shanghai Engineering Research Center of Molecular Therapeutics & New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200062, China.
Yuanzhe HuaCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Chuanxi ZhangDepartment of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
Bohuan FangDepartment of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
Xiang LongCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Yue PanShanghai Engineering Research Center of Molecular Therapeutics & New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200062, China.
Bei GaoSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
John Z H ZhangShanghai Engineering Research Center of Molecular Therapeutics & New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200062, China.
Lijun LiCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Hui NiCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China. nihui@jmu.edu.cn.
Lujia ZhangShanghai Engineering Research Center of Molecular Therapeutics & New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200062, China. Ljzhang@chem.ecnu.edu.cn.ORCID http://orcid.org/0000-0003-3566-917X
Jimei University · CNEast China Normal University · CNNew York University Shanghai · CNShanghai Jiao Tong University · CNUniversity of Shanghai for Science and Technology · CN

Funding

National Key R&D Program of China 2021YFC2102400National Natural Science Foundation of China 22208106National Natural Science Foundation of China U1805235Natural Science Foundation of Shanghai 21ZR1416500
6 · The paper itself

Abstract

The flavonoid naringenin is abundantly present in pomelo peels, and the unprocessed naringenin in wastes is not friendly for the environment once discarded directly. Fortunately, the hydroxylated product of eriodictyol from naringenin exhibits remarkable antioxidant and anticancer properties. The P450s was suggested promising for the bioconversion of the flavonoids, but less naturally existed P450s show hydroxylation activity to C3' of the naringenin. By well analyzing the catalytic mechanism and the conformations of the naringenin in P450, we proposed that the intermediate Cmpd I ((porphyrin)Fe = O) is more reasonable as key conformation for the hydrolyzation, and the distance between C3'/C5' of naringenin to the O atom of CmpdI determines the hydroxylating activity for the naringenin. Thus, the "flying kite model" that gradually drags the C-H bond of the substrate to the O atom of CmpdI was put forward for rational design. With ab initio design, we successfully endowed the self-sufficient P450-BM3 hydroxylic activity to naringenin and obtained mutant M5-5, with k

Indexed as

CitrusFlavanonesFlavonoidsHydroxylationeriodictyolFlavanonesFlavonoidsnaringeninEriodictyolFlying kite modelNaringeninP450BM3

Identifiers

PMID38189953
PMCPMC13498082
OpenAlexW4390669989

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.