Evidence mapPaperPMID 38201280Full record

ArticleCells2023

Metabolic Changes during In Vivo Maturation of PSC-Derived Skeletal Myogenic Progenitors.

Phablo Abreu, Bayardo I Garay, Travis Nemkov, Aline M S Yamashita, Rita C R Perlingeiro

Open access · goldAbstract read
In one paragraph

Article in Cells, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Phablo AbreuLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
Bayardo I GarayLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-1458-6095
Travis NemkovDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.ORCID 0000-0001-8566-7119
Aline M S YamashitaLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-8195-0416
Rita C R PerlingeiroLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0001-9412-1118
University of Minnesota · USUniversity of Colorado Anschutz Medical Campus · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM008244 · UNIVERSITY OF MINNESOTA TWIN CITIES · 1988 to 2005
$1.9M
Targeting Dystroglycanopathies using Pluripotent-derived Myogenic ProgenitorsR01AR081882 · NIAMS · UNIVERSITY OF MINNESOTA · 2023 to 2025
$1.0M
Preclinical studies of pluripotent stem cell-derived myogenic progenitors in non-human primatesR01AR078624 · UNIVERSITY OF MINNESOTA · 2025 to 2025
$510k
Predoctoral Training of NeuroscientistsT32NS105604 · UNIVERSITY OF MINNESOTA · 2025 to 2025
$413k
Skeletal Muscle Regeneration from Pluripotent Stem CellsR01AR078571 · UNIVERSITY OF MINNESOTA · 2025 to 2025
$409k
NHLBI NIH HHS F30 HL151138NIAMS NIH HHS R01 AR078571NIAMS NIH HHS R01 AR078571, AR078624, and AR081882NIAMS NIH HHS R01 AR078624NIAMS NIH HHS R01 AR081882NIGMS NIH HHS T32 GM008244NINDS NIH HHS T32 NS105604
6 · The paper itself

Abstract

In vitro-generated pluripotent stem cell (PSC)-derived Pax3-induced (iPax3) myogenic progenitors display an embryonic transcriptional signature, but upon engraftment, the profile of re-isolated iPax3 donor-derived satellite cells changes toward similarity with postnatal satellite cells, suggesting that engrafted PSC-derived myogenic cells remodel their transcriptional signature upon interaction within the adult muscle environment. Here, we show that engrafted myogenic progenitors also remodel their metabolic state. Assessment of oxygen consumption revealed that exposure to the adult muscle environment promotes overt changes in mitochondrial bioenergetics, as shown by the substantial suppression of energy requirements in re-isolated iPax3 donor-derived satellite cells compared to their in vitro-generated progenitors. Mass spectrometry-based metabolomic profiling further confirmed the relationship of engrafted iPax3 donor-derived cells to adult satellite cells. The fact that in vitro-generated myogenic progenitors remodel their bioenergetic signature upon in vivo exposure to the adult muscle environment may have important implications for therapeutic applications.

Indexed as

Pluripotent Stem CellsSatellite Cells, Skeletal MuscleAdultHumansMetabolomicsMusclesOxygen Consumptionin vitroin vivometabolismmyogenic progenitorspluripotent stem cellssatellite cells

Identifiers

PMID38201280
PMCPMC10778145
OpenAlexW4390405748

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.