Evidence map›Paper›PMID 38212600›Full record

ArticleScientific reports2024

Lactate-induced autophagy activation: unraveling the therapeutic impact of high-intensity interval training on insulin resistance in type 2 diabetic rats.

Hossein Pirani, Afsaneh Soltany, Maryam Hossein Rezaei, Adeleh Khodabakhshi Fard, Rohollah Nikooie, Kimya Khoramipoor, Karim Chamari, Kayvan Khoramipour

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
6.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 2 countries.

Hossein PiraniDepartment of Basic Sciences, Chabahar Maritime University, Chabahar, Iran.
Afsaneh SoltanyDepartment of Biology, Faculty of Science, University of Shiraz, Shiraz, Iran.
Maryam Hossein RezaeiDepartment of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Bahonar University, Kerman, Iran.
Adeleh Khodabakhshi FardDepartment on Nutrition, Faculty of Public Health, Kerman University of Medical Sciences, Kerman, Iran.
Rohollah NikooieDepartment of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Bahonar University, Kerman, Iran.
Kimya KhoramipoorDepartment of Nursing, Faculty of Nursing and Midwifery, Kurdistan University of Medical Sciences, Kurdistan, Iran.
Karim ChamariHigher Institute of Sport and Physical Education, ISSEP Ksar Said, Manouba University, Manouba, Tunisia.
Kayvan KhoramipourNeuroscience Research Center, Institute of Neuropharmacology and Department of Physiology and Pharmacology, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran. k.khoramipour@gmail.com.
Kerman University of Medical Sciences · IRShahid Bahonar University of Kerman · IRChabahar Maritime University · IRKurdistan University of Medical Sciences · IRManouba University · TNShiraz University · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Impaired autophagy is a hallmark of diabetes. The current study proposed to investigate if high intensity interval training (HIIT) induced lactate accumulation could stimulate autophagy in type 2 diabetic male rats. 28 male Wistar rats were randomly assigned into four groups: Healthy Control (CO), Diabetes Control (T2D), Exercise (EX), and Diabetes + Exercise (T2D + EX). Diabetes was induced by feeding high-fat diet and administrating single dose of streptozotocin (35 mg/kg). After becoming diabetic, the animals in the exercise groups (EX and T2D + EX) performed an eight-week HIIT (4-10 interval, 80-100% Vmax, 5 days per week). Serum levels of lactate, glucose and insulin as well as the levels of lactate, pyruvate, lactate transporter monocarboxylate transporter 1 (MCT1), phosphorylated mitogen-activated protein kinases (p-MAP 1 and 2), phosphorylated extracellular signal-regulated protein kinases 1 and 2 (p-ERK 1 and 2), mammalian target of rapamycin (p-mTOR), ribosomal protein S6 kinase beta-1 (p-70S6k), p90 ribosomal S6 kinases (p-90RSK), autophagy related 7 (ATG7), Beclin-1, microtubule-associated protein 1A/1B, and 2A/2B -light chain 3 levels (LC3-I), (LC3- II), (LC3I/LC3II) in soleus muscle were measured. Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) and serum glucose was lower in T2D + EX compared to T2D group (P < 0.0001). While serum and soleus muscle levels of lactate was not different between T2D and T2D + Ex, the levels of Pyruvate (P < 0.01), MCT1, p-ERK1/2, p-mTOR, p70S6k, P-90RSK, ATG7, LC3-II, and LC3-II/LC3I ratios were higher in T2D + EX compared to T2D group (P < 0.0001). We concluded that eight weeks of high-intensity interval training could activated ERK/P90SRK while inhibiting mTOR/P70S6K signaling pathway in lactate dependent manner. It means increased autophagy which resulted in improve insulin resistance (IR) and reduce blood glucose.

Indexed as

Diabetes Mellitus, ExperimentalDiabetes Mellitus, Type 2High-Intensity Interval TrainingInsulin ResistanceAnimalsAutophagyGlucoseInsulinLactic AcidMaleMammalsPyruvatesRatsRats, WistarRibosomal Protein S6 Kinases, 70-kDaTOR Serine-Threonine KinasesGlucoseInsulinLactic AcidPyruvatesRibosomal Protein S6 Kinases, 70-kDaTOR Serine-Threonine Kinases

Identifiers

PMID38212600
PMCPMC10784291
OpenAlexW4390752659

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.