Evidence map›Paper›PMID 38252383›Full record

ArticleMolecular neurobiology2024

A Panel of miRNA Biomarkers Common to Serum and Brain-Derived Extracellular Vesicles Identified in Mouse Model of Amyotrophic Lateral Sclerosis.

Natasha Vassileff, Jereme G Spiers, John D Lee, Trent M Woodruff, Esmaeil Ebrahimie, Manijeh Mohammadi Dehcheshmeh, Andrew F Hill, Lesley Cheng

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular neurobiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

Natasha VassileffDepartment of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia.ORCID http://orcid.org/0000-0001-6554-4496
Jereme G SpiersClear Vision Research, Eccles Institute of Neuroscience, John Curtin School of Medical Research, College of Health and Medicine, The Australian National University, Acton, ACT, Australia.ORCID http://orcid.org/0000-0001-5872-8983
John D LeeSchool of Biomedical Sciences, The University of Queensland, St. Lucia, Australia.ORCID http://orcid.org/0000-0002-9976-7396
Trent M WoodruffSchool of Biomedical Sciences, The University of Queensland, St. Lucia, Australia.ORCID http://orcid.org/0000-0003-1382-911X
Esmaeil EbrahimieGenomics Research Platform, School of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, VIC, 3000, Australia.ORCID http://orcid.org/0000-0002-4431-2861
Manijeh Mohammadi DehcheshmehSchool of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, SA, 5371, Australia.ORCID http://orcid.org/0000-0002-8019-4081
Andrew F HillDepartment of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia.ORCID http://orcid.org/0000-0001-5581-2354
Lesley ChengDepartment of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia. l.cheng@latrobe.edu.au.ORCID http://orcid.org/0000-0002-8075-6144
La Trobe University · AUThe University of Queensland · AUAustralian National University · AUThe University of Adelaide · AUThe University of Melbourne · AU

Funding

National Health and Medical Research Council GNT1041413
6 · The paper itself

Abstract

Amyotrophic lateral sclerosis (ALS) is a progressive motor neuron disease characterised by the deposition of aggregated proteins including TAR DNA-binding protein 43 (TDP-43) in vulnerable motor neurons and the brain. Extracellular vesicles (EVs) facilitate the spread of neurodegenerative diseases and can be easily accessed in the bloodstream. This study aimed to identify a panel of EV miRNAs that can capture the pathology occurring in the brain and peripheral circulation. EVs were isolated from the cortex (BDEVs) and serum (serum EVs) of 3 month-old and 6-month-old TDP-43*Q331K and TDP-43*WT mice. Following characterisation and miRNA isolation, the EVs underwent next-generation sequencing where 24 differentially packaged miRNAs were identified in the TDP-43*Q331K BDEVs and 7 in the TDP-43*Q331K serum EVs. Several miRNAs, including miR-183-5p, were linked to ALS. Additionally, miR-122-5p and miR-486b-5p were identified in both panels, demonstrating the ability of the serum EVs to capture the dysregulation occurring in the brain. This is the first study to identify miRNAs common to both the serum EVs and BDEVs in a mouse model of ALS.

Indexed as

Amyotrophic Lateral SclerosisBiomarkersBrainDisease Models, AnimalExtracellular VesiclesMicroRNAsAnimalsDNA-Binding ProteinsMaleMiceMice, Inbred C57BLMice, TransgenicBiomarkersDNA-Binding ProteinsMicroRNAsALSAmyotrophic lateral sclerosisBDEVExtracellular vesiclesmiRNATDP-43

Identifiers

PMID38252383
PMCPMC11249427
OpenAlexW4391107216

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.