Evidence map›Paper›PMID 38255802›Full record

ArticleInternational journal of molecular sciences2024

Evaluation of the LDN-0060609 PERK Inhibitor as a Selective Treatment for Primary Open-Angle Glaucoma: An In Vitro Study on Human Retinal Astrocytes.

Wioletta Rozpędek-Kamińska, Grzegorz Galita, Kamil Saramowicz, Zuzanna Granek, Julia Barczuk, Natalia Siwecka, Dariusz Pytel, Ireneusz Majsterek

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Wioletta Rozpędek-KamińskaDepartment of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-419 Lodz, Poland.ORCID 0000-0001-9206-1203
Grzegorz GalitaDepartment of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-419 Lodz, Poland.ORCID 0000-0002-1559-2796
Kamil SaramowiczDepartment of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-419 Lodz, Poland.ORCID 0009-0000-0395-5986
Zuzanna GranekDepartment of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-419 Lodz, Poland.ORCID 0000-0001-5765-1654
Julia BarczukDepartment of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-419 Lodz, Poland.ORCID 0000-0003-4708-9164
Natalia SiweckaDepartment of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-419 Lodz, Poland.ORCID 0000-0002-0308-580X
Dariusz PytelDepartment of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29425, USA.ORCID 0000-0002-3849-7716
Ireneusz MajsterekDepartment of Clinical Chemistry and Biochemistry, Medical University of Lodz, 90-419 Lodz, Poland.
Medical University of Lodz · PLMedical University of South Carolina · US

Funding

Medical University of Lodz 503/1-156-07/503-11-001Medical University of Lodz 564/1-000-00/564-20-064National Science Center 2016/21/B/NZ5/01411National Science Center 2021/43/O/NZ5/02068
6 · The paper itself

Abstract

The term glaucoma encompasses various neurodegenerative eye disorders, among which the most common is primary open-angle glaucoma (POAG). Recently, the essential role of human retinal astrocytes (HRA) in glaucoma progression has been placed in the spotlight. It has been found that placing the endoplasmic reticulum (ER) under stress and activating PERK leads to apoptosis of HRA cells, which inhibits their neuroprotective effect in the course of glaucoma. Therefore, the aim of the present study was to evaluate the effectiveness of the small-molecule PERK inhibitor LDN-0060609 in countering ER stress conditions induced in HRA cells in vitro. The activity of LDN-0060609 was studied in terms of protein and mRNA expression, cytotoxicity, genotoxicity, caspase-3 level and cell cycle progression. LDN-0060609 at 25 μM proved to be a potent inhibitor of the major PERK substrate, p-eIF2α (49% inhibition). The compound markedly decreased the expression of pro-apoptotic ER stress-related genes (

Indexed as

GlaucomaGlaucoma, Open-AngleAstrocytesCaspase 3HumansResearch DesignCaspase 3eIF2αendoplasmic reticulum stressglaucomaglaucoma treatmentPERKPERK inhibitorunfolded protein response

Identifiers

PMID38255802
PMCPMC10815359
OpenAlexW4390617581

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.