SynthesisBMC oral health2024
Extensive comparison of salivary collection, transportation, preparation, and storage methods: a systematic review.
Synthesis in BMC oral health, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence of disability in inflammatory bowel disease: a systematic review and meta-analysis.Inflammatory bowel diseases · 2026Pooled it
- Best practices for using biological data in psychological research.Journal of mood and anxiety disorders · 2026Review
- On the correlation of tryptophan and its kynurenine pathway metabolites level in blood, saliva and urine in systemic sclerosis patients.Rheumatology international · 2026Article
- Relationship Between Salivary and Serum Cardiac Troponin I in Patients Undergoing Cardiac Surgery: A Prospective Longitudinal Observational Study.Diagnostics (Basel, Switzerland) · 2026Article
- Saliva-based monitoring of chronic kidney disease: comparative evaluation of three collection methods for creatinine, urea, calcium, and PTH.Scientific reports · 2026Article
- Translational Assessment of Omics Approaches in Endometriosis: Bridging Molecular Discovery with Clinical Utility.International journal of molecular sciences · 2026Review
- Sex-specific immune-brain coupling in hippocampal circuits and Alzheimer's disease vulnerability.Research square · 2026Article
- Saliva as a Matrix for Primary Care: Feasibility and Scoping of Its Use for Assessment of Nutrition and Inflammation.Advances in nutrition (Bethesda, Md.) · 2026Review
- A Minimally Invasive LC-MS/MS Approach for Assessing Endocannabinoids in Saliva and Capillary Blood Microsamples.Biosensors · 2026Article
- Minor salivary flow as a diagnostic tool for screening hyposalivation in Medication-Induced Xerostomia.BMC oral health · 2026Article
- Impact of smoking and periodontitis on salivary IL-39, IL-41, IL-1β, and TNF-α levels: a cross-sectional analysis.BMC oral health · 2026Article
- Salivary Antioxidant and Peroxidase Activity as a Marker of Steroid Hormone Receptor Expression in Breast Cancer.International journal of molecular sciences · 2026Article
- Salivary biomarkers in oral cancer diagnosis: advancing conventional treatment strategies.Military Medical Research · 2026Review
- Analysis of Respiratory Microbiota Characteristics in Patients with COPD and High-Risk Populations Using 16S rRNA Technology.International journal of chronic obstructive pulmonary disease · 2026Article
- Volumetric Absorptive Microsampling of Saliva for Pharmacokinetic Evaluation of Mycophenolic Acid and Its Glucuronide Metabolite in Pediatric Renal Transplant Recipients: Bioanalytical Method Validation and Clinical Feasibility Evaluation.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Flight-associated cortisol dynamics and subjective assessments of pain and stress in fighter pilots: an exploratory study.European journal of applied physiology · 2025Article
- Salivary Biomarkers in Periodontal Disease: Revolutionizing Early Detection and Precision Dentistry.Molecular diagnosis & therapy · 2025Review
- Immunological Status of Saliva in Children with Asthma by Disease Severity.International journal of clinical pediatric dentistry · 2025Article
- Advancements and Challenges in Salivary Metabolomics for Early Detection and Monitoring of Systemic Diseases.MedComm · 2025Review
- Oral nitrate-reducing capacity and aerobic fitness are impaired in older individuals with heart failure with reduced ejection fraction.American journal of physiology. Heart and circulatory physiology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman saliva as a bodily fluid-similar to blood-is utilized for diagnostic purposes. Unlike blood sampling, collecting saliva is non-invasive, inexpensive, and readily accessible. There are no previously published systematic reviews regarding different collection, transportation, preparation, and storage methods for human saliva.
designThis study has been prepared and organized according to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) 2020 guidelines. This systematic review has been registered at PROSPERO (Registration ID: CRD42023415384). The study question according to the PICO format was as followed: Comparison of the performance (C) of different saliva sampling, handling, transportation, and storage techniques and methods (I) assessed for analyzing stimulated or unstimulated human saliva (P and O). An electronic search was executed in Scopus, Google Scholar, and PubMed.
resultsTwenty-three descriptive human clinical studies published between 1995 and 2022 were included. Eight categories of salivary features and biomarkers were investigated (i.e., salivary flow rate, total saliva quantity, total protein, cortisol, testosterone, DNA quality and quantity, pH and buffering pH). Twenty-two saliva sampling methods/devices were utilized. Passive drooling, Salivette®, and spitting were the most utilized methods. Sampling times with optimum capabilities for cortisol, iodine, and oral cancer metabolites are suggested to be 7:30 AM to 9:00 AM, 10:30 AM to 11:00 AM, and 14:00 PM to 20:00 PM, respectively. There were 6 storage methods. Centrifuging samples and storing them at -70 °C to -80 °C was the most utilized storage method. For DNA quantity and quality, analyzing samples immediately after collection without centrifuging or storage, outperformed centrifuging samples and storing them at -70 °C to -80 °C. Non-coated Salivette® was the most successful method/device for analyzing salivary flow rate.
conclusionIt is highly suggested that scientists take aid from the reported categorized outcomes, and design their study questions based on the current voids for each method/device.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.