Evidence map›Paper›PMID 38315364›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates.

Federico Urbano-Munoz, Caitlyn E Orne, Mary N Burtnick, Paul J Brett

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Federico Urbano-MunozDepartment of Microbiology and Immunology, University of Nevada, Reno School of Medicine, Reno, NV, USA.
Caitlyn E OrneDepartment of Microbiology and Immunology, University of Nevada, Reno School of Medicine, Reno, NV, USA.
Mary N BurtnickDepartment of Microbiology and Immunology, University of Nevada, Reno School of Medicine, Reno, NV, USA.
Paul J BrettDepartment of Microbiology and Immunology, University of Nevada, Reno School of Medicine, Reno, NV, USA. pbrett@med.unr.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reductive amination is a relatively simple and convenient strategy for coupling purified polysaccharides to carrier proteins. Following their synthesis, glycoconjugates can be used to assess the protective capacity of specific microbial polysaccharides in animal models of infection and/or to produce polyclonal antiserum and monoclonal antibodies for a variety of immune assays. Here, we describe a reproducible method for chemically activating the 6-deoxyheptan capsular polysaccharide (CPS) from Burkholderia pseudomallei and covalently linking it to recombinant CRM197 diphtheria toxin mutant (CRM197) to produce the glycoconjugate, CPS-CRM197. Similar approaches can also be used to couple other types of polysaccharides to CRM197 with little to no modification of the protocol.

Indexed as

Burkholderia pseudomalleiPolysaccharidesAminationAnimalsGlycoconjugatesVaccines, ConjugateGlycoconjugatesPolysaccharidesVaccines, ConjugateBurkholderia pseudomalleiBurkholderia thailandensisCapsular polysaccharideCarrier proteinCRM197GlycoconjugateOxidationReductive amination

Identifiers

PMID38315364

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.