Oxygen Assessment in Tumors In Vivo Using Phosphorescence Lifetime Imaging Microscopy.

Anastasia D Komarova, Vladislav I Shcheslavskiy, Anton A Plekhanov, Marina A Sirotkina, Leonid N Bochkarev, Marina V Shirmanova

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Anastasia D Komarova *Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod, Russia.

Vladislav I Shcheslavskiy *Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod, Russia. vis@becker-hickl.de.

Anton A PlekhanovInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod, Russia.

Marina A SirotkinaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod, Russia.

Leonid N BochkarevG. A. Razuvaev Institute of Metallo-organic Chemistry, Russian Academy of Sciences, Nizhny Novgorod, Russia.

Marina V ShirmanovaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The oxygen level in a tumor is a crucial factor for its development and response to therapies. Phosphorescence lifetime imaging (PLIM) with the use of phosphorescent oxygen probes is a highly sensitive, noninvasive optical technique for the assessment of molecular oxygen in living cells and tissues. Here, we present a protocol for microscopic mapping of oxygen distribution in a mouse tumor model in vivo. We demonstrate that PLIM microscopy, in combination with an Ir(III)-based probe, enables visualization of cellular-level heterogeneity of tumor oxygenation.

Indexed as

NeoplasmsRadiationAnimalsDisease Models, AnimalMiceMicroscopyOxygenOxygenIn vivoOxygenationPhosphorescence decayPhosphorescence lifetime imaging PLIMPhosphorescent oxygen probeTumor

Identifiers

PMID38319571

DOI10.1038/s41598-020-68631-w

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.