Evidence map›Paper›PMID 38328036›Full record

ArticlebioRxiv : the preprint server for biology2024

CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization.

Viacheslav Serbynovskyi, Jing Wang, Eugene Yd Chua, Aygul Ishemgulova, Lambertus M Alink, William C Budell, Jake D Johnston, Charlie Dubbeldam, Fabio A Gonzalez, Sharon Rozovsky and 3 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Viacheslav SerbynovskyiSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0000-0002-8789-6537
Jing WangSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0000-0003-1148-9457
Eugene Yd ChuaSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0000-0003-1224-2157
Aygul IshemgulovaSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0000-0003-0608-6530
Lambertus M AlinkSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0009-0005-5271-3250
William C BudellSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0000-0002-5538-668X
Jake D JohnstonSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0000-0003-3060-7738
Charlie DubbeldamSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0009-0003-7821-5087
Fabio A GonzalezDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.ORCID 0000-0002-6123-2062
Sharon RozovskyDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.ORCID 0000-0003-4902-0777
Edward T EngSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0000-0002-8014-7269
Alex de MarcoSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0000-0001-6238-5653
Alex J NobleSimons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.ORCID 0000-0001-8634-2279
New York Structural Biology Center · USUniversity of Delaware · US

Funding

NCCAT: National Center for CryoEM Access and Training- Supplement for Windows 10 and FFIU24GM129539 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2018 to 2023
$53.9M
Selenoproteins in the ER-associated protein degradation pathwayR01GM121607 · NIGMS · UNIVERSITY OF DELAWARE · PI ROZOVSKY, SHARON · 2017 to 2021
$1.1M
NIGMS NIH HHS R01 GM121607NIGMS NIH HHS U24 GM129539
6 · The paper itself

Abstract

CryoEM democratization is hampered by access to costly plunge-freezing supplies. We introduce methods, called CryoCycle, for reliably blotting, vitrifying, and reusing clipped cryoEM grids. We demonstrate that vitreous ice may be produced by plunging clipped grids with purified proteins into liquid ethane and that clipped grids may be reused several times for different protein samples. Furthermore, we demonstrate the vitrification of thin areas of cells prepared on gold-coated, pre-clipped grids.

Identifiers

PMID38328036
PMCPMC10849629
OpenAlexW4391175117

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.