Evidence map›Paper›PMID 38332379›Full record

ArticlePlanta2024

Temporal profiling of physiological, histological, and transcriptomic dissection during auxin-induced adventitious root formation in tetraploid Robinia pseudoacacia micro-cuttings.

Saleem Uddin, Muhammad Zeeshan Munir, Eduardo Larriba, José Manuel Pérez-Pérez, Sadia Gull, Tariq Pervaiz, Umer Mahmood, Zahid Mahmood, Yuhan Sun, Yun Li

Abstract read
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In one paragraph

Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
4.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 8 institutions in 5 countries.

Saleem UddinState Key Laboratory of Tree Genetics and Breeding, Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, People's Republic of China.
Muhammad Zeeshan MunirState Key Laboratory of Tree Genetics and Breeding, Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, People's Republic of China.
Eduardo LarribaInstituto de Bioingeniería, Universidad Miguel Hernández de Elche, Alicante, Spain.
José Manuel Pérez-PérezInstituto de Bioingeniería, Universidad Miguel Hernández de Elche, Alicante, Spain.
Sadia GullDepartment of Horticulture, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China.
Tariq PervaizDepartment of Botany and Plant Sciences, University of California Riverside, Riverside, CA, 22963, USA.
Umer MahmoodDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.
Zahid MahmoodCrop Sciences Institute, National Agricultural Research Centre, Islamabad, 44000, Pakistan.
Yuhan SunState Key Laboratory of Tree Genetics and Breeding, Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, People's Republic of China. syh831008@163.com.
Yun LiState Key Laboratory of Tree Genetics and Breeding, Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, People's Republic of China. yunli@bjfu.edu.cn.ORCID http://orcid.org/0000-0002-8426-565X
Beijing Forestry University · CNUniversitat de Miguel Hernández d'Elx · ESPakistan Agricultural Research Council · PKPeking University · CNSouthwest University · CNSwedish University of Agricultural Sciences · SEUniversity of California, Riverside · USYangzhou University · CN

Funding

Ministerio de Ciencia e Innovación BIO2015-64255-RMinisterio de Ciencia e Innovación RTI2018-096505-B-I00National Key R&D Program of China 2017YFD0600503National Natural Science Foundation of China 31971675
6 · The paper itself

Abstract

MAIN

conclusionOptimal levels of indole-3-butyric acid (IBA) applied at the stem base promote adventitious root (AR) initiation and primordia formation, thus promoting the rooting of leafy micro-cuttings of tetraploid Robinia pseudoacacia. Tetraploid Robinia pseudoacacia L. is a widely cultivated tree in most regions of China that has a hard-rooting capability, propagated by stem cuttings. This study utilizes histological, physiological, and transcriptomic approaches to explore how root primordia are induced after indole butyric acid (IBA) treatment of micro-cuttings. IBA application promoted cell divisions in some cells within the vasculature, showing subcellular features associated with adventitious root (AR) founder cells. The anatomical structure explicitly showed that AR initiated from the cambium layer and instigate the inducible development of AR primordia. Meanwhile, the hormone data showed that similar to that of indole-3-acetic acid, the contents of trans-zeatin and abscisic acid peaked at early stages of AR formation and increased gradually in primordia formation across the subsequent stages, suggesting their indispensable roles in AR induction. On the contrary, 24-epibrassinolide roughly maintained at extremely high levels during primordium initiation thoroughly, indicating its presence was involved in cell-specific reorganization during AR development. Furthermore, antioxidant activities transiently increased in the basal region of micro-cuttings and may serve as biochemical indicators for distinct rooting phases, potentially aiding in AR formation. Transcriptomic analysis during the early stages of root formation shows significant downregulation of the abscisic acid and jasmonate signaling pathways, while ethylene and cytokinin signaling seems upregulated. Network analysis of genes involved in carbon metabolism and photosynthesis indicates that the basal region of the micro-cuttings undergoes rapid reprogramming, which results in the breakdown of sugars into pyruvate. This pyruvate is then utilized to fuel the tricarboxylic acid cycle, thereby sustaining growth through aerobic respiration. Collectively, our findings provide a time-course morphophysiological dissection and also suggest the regulatory role of a conserved auxin module in AR development in these species.

Indexed as

Abscisic AcidRobiniaGene Expression ProfilingIndoleacetic AcidsPlant RootsPyruvatesTetraploidyAbscisic AcidIndoleacetic AcidsPyruvatesAdventitious root inductionGene expressionHistomorphologyMicrocuttingsPlant hormoneTetraploid Robinia pseudoacaciaTime-course RNA-SeqTranscriptomeWoody plant propagation

Identifiers

PMID38332379
OpenAlexW4391657977

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.