Evidence map›Paper›PMID 38345101›Full record

ArticleJournal of cell science2024

Culture substrate stiffness impacts human myoblast contractility-dependent proliferation and nuclear envelope wrinkling.

Jo Nguyen, Lu Wang, Wen Lei, Yechen Hu, Nitya Gulati, Carolina Chavez-Madero, Henry Ahn, Howard J Ginsberg, Roman Krawetz, Matthias Brandt and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.3field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Aberrant evoked calcium signaling and nAChR cluster morphology in aFrontiers in cell and developmental biology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Jo NguyenInstitute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3E2, Canada.ORCID 0000-0002-8145-4930
Lu WangDonnelly Centre, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Wen LeiDonnelly Centre, University of Toronto, Toronto, ON, M5S 3E1, Canada.
Yechen HuDepartment of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada.
Nitya GulatiInstitute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3E2, Canada.
Carolina Chavez-MaderoInstitute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3E2, Canada.
Henry AhnDepartment of Surgery, University of Toronto, Toronto, ON, M5G 2C4, Canada.
Howard J GinsbergDepartment of Surgery, University of Toronto, Toronto, ON, M5G 2C4, Canada.
Roman KrawetzMcCaig Institute, University of Calgary, Calgary, AB, T2N 4Z6, Canada.
Matthias BrandtInstitute of Cell Biology, Center for Molecular Biology of Inflammation, University Münster, 48149 Münster, Germany.ORCID 0000-0003-1730-6228
Timo BetzThird Institute of Physics - Biophysics, Georg August University Göttingen, 37077 Göttingen, Germany.ORCID 0000-0002-1548-0655
Penney M GilbertInstitute of Biomedical Engineering, University of Toronto, Toronto, ON, M5S 3E2, Canada.ORCID 0000-0001-5509-9616
University of Toronto · CASt. Michael's Hospital · CAUniversity of Calgary · CAUniversity of Göttingen · DE

Funding

Canada Research Chairs 950-231201Connaught International ScholarshipConnaught International Scholarship JN/2019-2023Deutsche Forschungsgemeinschaft BE 6270/2-1Human Frontier Science Program RGP0018/2017Human Frontiers Science Program RGP0018/2017Natural Sciences and Engineering Research Council of Canada RGPIN-2019-07144University of Toronto
6 · The paper itself

Abstract

Understanding how biophysical and biochemical microenvironmental cues together influence the regenerative activities of muscle stem cells and their progeny is crucial in strategizing remedies for pathological dysregulation of these cues in aging and disease. In this study, we investigated the cell-level influences of extracellular matrix (ECM) ligands and culture substrate stiffness on primary human myoblast contractility and proliferation within 16 h of plating and found that tethered fibronectin led to stronger stiffness-dependent responses compared to laminin and collagen. A proteome-wide analysis further uncovered cell metabolism, cytoskeletal and nuclear component regulation distinctions between cells cultured on soft and stiff substrates. Interestingly, we found that softer substrates increased the incidence of myoblasts with a wrinkled nucleus, and that the extent of wrinkling could predict Ki67 (also known as MKI67) expression. Nuclear wrinkling and Ki67 expression could be controlled by pharmacological manipulation of cellular contractility, offering a potential cellular mechanism. These results provide new insights into the regulation of human myoblast stiffness-dependent contractility response by ECM ligands and highlight a link between myoblast contractility and proliferation.

Indexed as

Extracellular MatrixNuclear EnvelopeCell ProliferationHumansKi-67 AntigenMyoblastsKi-67 AntigenCell contractilityExtracellular matrixMechanobiologyMyoblastNuclear envelopeSubstrate stiffness

Identifiers

PMID38345101
PMCPMC11033523
OpenAlexW4391747149

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.