Evidence map›Paper›PMID 38369441›Full record

ArticleInternational dental journal2024

Photosensitive Hydrogels Encapsulating DPSCs and AgNPs for Dental Pulp Regeneration.

Yan He, Yanni Zhang, Fengting Hu, Min Chen, Ben Wang, Yejian Li, Haichao Xu, Na Dong, Chen Zhang, Yunfan Hu and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International dental journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
3.9field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Yan HeSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China; Tianyou Hospital, Wuhan University of Science and Technology, Wuhan, China.
Yanni ZhangSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Fengting HuSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Min ChenSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Ben WangSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Yejian LiSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Haichao XuSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Na DongOujiang Laboratory, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Chen ZhangSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Yunfan HuSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Zhiqiang LinSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Youjian PengCenter of Regenerative Medicine, Department of Stomatology, Renmin Hospital of Wuhan University, Wuhan, China.
Qingsong YeSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China; Center of Regenerative Medicine, Department of Stomatology, Renmin Hospital of Wuhan University, Wuhan, China. Electronic address: qingsongye@whu.edu.cn.
Lihua LuoSchool and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China. Electronic address: luolihua81@126.com.
Wenzhou Medical University · CNWuhan University · CNWuhan University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivePulp regeneration with bioactive dentin-pulp complex has been a research hotspot in recent years. Stem cell therapy provided an interest strategy to regenerate the dental-pulp complex. Hence, this study aimed to evaluate the effects of photosensitive gelatin methacrylate (GelMA) hydrogel encapsulating dental pulp stem cells (DPSCs) and silver nanoparticles (AgNPs) for dental pulp regeneration in vitro.

methodsFirst, the AgNPs@GelMA hydrogels were prepared by lithium phenyl-2,4,6-trimethyl-benzoyl phosphinate (LAP) initiation via blue-light emitting diode light. The physical and chemical properties of AgNPs@GelMA hydrogels were comprehensively analysed via scanning electron microscopy (SEM), and mechanical characterisation, such as swelling ability, degradation properties, and AgNP release profile. Then, AgNPs@GelMA hydrogels encapsulated DPSCs were used to establish an AgNPs@GelMA biomimetic complex, further analysing its biocompatibility, antibacterial properties, and angiogenic capacity in vitro.

resultsThe results indicated that GelMA hydrogels demontrated optimal characteristics with a monomer:LAP ratio of 16:1. The physico-chemical properties of AgNPs@GelMA hydrogels did not change significantly after loading with AgNPs. There was no significant difference in AgNP release rate amongst different concentrations of AgNPs@GelMA hydrogels. Fifty to 200 μg/mL AgNPs@GelMA hydrogels could disperse E faecalis biofilm and reduce its metabolic activity . Furthermore, cell proliferation was arrested in 100 and 200 μg/mL AgNPs@GelMA hydrogels. The inhibition of 50 μg/mL AgNPs@GelMA hydrogels on E faecalis biofilm was above 50%, and the cell viability of the hydrogels was higher than 90%. The angiogenesis assay indicated that AgNPs@GelMA hydrogels encapsulating DPSCs could induce the formation of capillary-like structures and express angiogenic markers CD31, vascular endothelial growth factor , and von willebrand factor (vWF) in vitro.

conclusionsResults of this study indicate that 50 μg/mL AgNPs@GelMA hydrogels encapsulating DPSCs had significant antibacterial properties and angiogenic capacity, which could provide a significant experimental basis for the regeneration of the dentin-pulp complex.

Indexed as

Dental PulpGelatinHydrogelsMetal NanoparticlesRegenerationSilverStem CellsAnti-Bacterial AgentsCell ProliferationHumansMethacrylatesMicroscopy, Electron, ScanningAnti-Bacterial AgentsGelatinHydrogelsMethacrylatesSilverAngiogenesisDental pulp regenerationDental pulp stem cellsHydrogelsSilver nanoparticles

Identifiers

PMID38369441
PMCPMC11287144
OpenAlexW4391899752

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.