Evidence mapPaperPMID 38388913Full record

ArticleJournal of translational medicine2024

Rab32 facilitates Schwann cell pyroptosis in rats following peripheral nerve injury by elevating ROS levels.

Jiayi Wang, Pin Chen, Guanjie Han, Yongjie Zhou, Xingdong Xiang, Mengxuan Bian, Lei Huang, Xiang Wang, Binfeng He, Shunyi Lu

Open access · goldAbstract read
In one paragraph

Article in Journal of translational medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Jiayi Wang *Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Pin Chen *Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Guanjie Han *Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Yongjie Zhou *Department of Interventional Radiology, Zhongshan Hospital, Fudan University, Shanghai, China.
Xingdong XiangDepartment of Rehabilitation, Zhongshan Hospital, Fudan University, Shanghai, China.
Mengxuan BianDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Lei HuangDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Xiang WangDepartment of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China. ndyfy10316@ncu.edu.cn.
Binfeng HeDepartment of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. ldhbf@126.com.ORCID 0009-0008-4734-8000
Shunyi LuDepartment of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China. lushunyi@suda.edu.cn.
Sun Yat-sen University · CNFudan University · CNArmy Medical University · CNNanchang University · CN

Funding

Post-doctoral Special Research Fund 202300470008
6 · The paper itself

Abstract

backgroundPeripheral nerve injury (PNI) is commonly observed in clinical practice, yet the underlying mechanisms remain unclear. This study investigated the correlation between the expression of a Ras-related protein Rab32 and pyroptosis in rats following PNI, and potential mechanisms have been explored by which Rab32 may influence Schwann cells pyroptosis and ultimately peripheral nerve regeneration (PNR) through the regulation of Reactive oxygen species (ROS) levels.

methodsThe authors investigated the induction of Schwann cell pyroptosis and the elevated expression of Rab32 in a rat model of PNI. In vitro experiments revealed an upregulation of Rab32 during Schwann cell pyroptosis. Furthermore, the effect of Rab32 on the level of ROS in mitochondria in pyroptosis model has also been studied. Finally, the effects of knocking down the Rab32 gene on PNR were assessed, morphology, sensory and motor functions of sciatic nerves, electrophysiology and immunohistochemical analysis were conducted to assess the therapeutic efficacy.

resultsSilencing Rab32 attenuated PNI-induced Schwann cell pyroptosis and promoted peripheral nerve regeneration. Furthermore, our findings demonstrated that Rab32 induces significant oxidative stress by damaging the mitochondria of Schwann cells in the pyroptosis model in vitro.

conclusionRab32 exacerbated Schwann cell pyroptosis in PNI model, leading to delayed peripheral nerve regeneration. Rab32 can be a potential target for future therapeutic strategy in the treatment of peripheral nerve injuries.

Indexed as

Peripheral Nerve InjuriesAnimalsCell ProliferationNerve RegenerationPyroptosisRatsRats, Sprague-DawleyReactive Oxygen SpeciesSchwann CellsSciatic NerveReactive Oxygen SpeciesPeripheral nerve regenerationPyroptosisRab32Schwann cells

Identifiers

PMID38388913
PMCPMC10885539
OpenAlexW4392032480

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.