Evidence mapPaperPMID 38397014Full record

ArticleInternational journal of molecular sciences2024

The Thiol Group Reactivity and the Antioxidant Property of Human Serum Albumin Are Controlled by the Joint Action of Fatty Acids and Glucose Binding.

Tamara Uzelac, Katarina Smiljanić, Marija Takić, Ivana Šarac, Gordana Oggiano, Milan Nikolić, Vesna Jovanović

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Tamara UzelacDepartment of Biochemistry and Centre of Excellence for Molecular and Food Sciences, University of Belgrade-Faculty of Chemistry (UBFC), Studentski trg 12-16, 11158 Belgrade, Serbia.
Katarina SmiljanićDepartment of Biochemistry and Centre of Excellence for Molecular and Food Sciences, University of Belgrade-Faculty of Chemistry (UBFC), Studentski trg 12-16, 11158 Belgrade, Serbia.ORCID 0000-0003-4774-8895
Marija TakićCentre of Research Excellence in Nutrition and Metabolism, Group for Nutrition and Metabolism, National Institute of Republic of Serbia, Institute for Medical Research, University of Belgrade, Tadeuša Košćuškog 1, 11000 Belgrade, Serbia.ORCID 0000-0001-8449-165X
Ivana ŠaracCentre of Research Excellence in Nutrition and Metabolism, Group for Nutrition and Metabolism, National Institute of Republic of Serbia, Institute for Medical Research, University of Belgrade, Tadeuša Košćuškog 1, 11000 Belgrade, Serbia.ORCID 0000-0003-1439-9561
Gordana OggianoCentre of Research Excellence in Nutrition and Metabolism, Group for Nutrition and Metabolism, National Institute of Republic of Serbia, Institute for Medical Research, University of Belgrade, Tadeuša Košćuškog 1, 11000 Belgrade, Serbia.ORCID 0000-0002-6248-4210
Milan NikolićDepartment of Biochemistry and Centre of Excellence for Molecular and Food Sciences, University of Belgrade-Faculty of Chemistry (UBFC), Studentski trg 12-16, 11158 Belgrade, Serbia.ORCID 0000-0003-0932-889X
Vesna JovanovićDepartment of Biochemistry and Centre of Excellence for Molecular and Food Sciences, University of Belgrade-Faculty of Chemistry (UBFC), Studentski trg 12-16, 11158 Belgrade, Serbia.
University of Belgrade · RS

Funding

The Ministry of Science, Technological Development and Innovation of the Republic of Serbia 451-03-47/2023-01/200015The Ministry of Science, Technological Development and Innovation of the Republic of Serbia 451-03-47/2023-01/200168
6 · The paper itself

Abstract

The binding of ubiquitous serum ligands (free fatty acids) to human serum albumin (HSA) or its glycation can affect thiol group reactivity, thus influencing its antioxidant activity. The effects of stearic acid (SA) and glucose binding on HSA structural changes and thiol group content and reactivity were monitored by fluoroscopy and the Ellman method during a 14-day incubation in molar ratios to HSA that mimic pathophysiological conditions. Upon incubation with 5 mM glucose, HSA glycation was the same as HSA without it, in three different HSA:SA molar ratios (HSA:SA-1:1-2-4). The protective effect of SA on the antioxidant property of HSA under different glucose regimes (5-10-20 mM) was significantly affected by molar ratios of HSA:SA. Thiol reactivity was fully restored with 5-20 mM glucose at a 1:1 HSA:SA ratio, while the highest thiol content recovery was in pathological glucose regimes at a 1:1 HSA:SA ratio. The SA affinity for HSA increased significantly (1.5- and 1.3-fold,

Indexed as

Serum Albumin, HumanSulfhydryl CompoundsAntioxidantsFatty AcidsHumansProtein BindingSerum AlbuminAntioxidantsFatty AcidsSerum AlbuminSerum Albumin, HumanSulfhydryl Compoundsantioxidant rolefatty acidsglucoseglycationhuman serum albuminthiol group content and reactivity

Identifiers

PMID38397014
PMCPMC10889162
OpenAlexW4391882837

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.