Evidence mapPaperPMID 38401045Full record

ArticleMolecular neurobiology2024

Transcriptomics and Metabolomics Unveil the Neuroprotection Mechanism of AnGong NiuHuang (AGNH) Pill Against Ischaemic Stroke Injury.

Liangliang Tian, Guangzhao Cao, Xiaotong Zhu, Lihan Wang, Jingyi Hou, Yi Zhang, He Xu, Lixia Wang, Shicong Wang, Chen Zhao and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular neurobiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Multi-omics reveals the protective mechanisms ofFrontiers in pharmacology · 2026
    Article
  2. [Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Liangliang Tian *Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Guangzhao Cao *Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Xiaotong ZhuInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Lihan WangExperimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Jingyi HouExperimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Yi ZhangInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
He XuInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Lixia WangInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Shicong WangFujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development, Zhangzhou, 363000, Fujian, China.
Chen ZhaoFujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development, Zhangzhou, 363000, Fujian, China.
Hongjun YangInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China. hongjun0420@vip.sina.com.
Jingjing ZhangInstitute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China. zjj4785@163.com.ORCID http://orcid.org/0000-0002-1349-9184
Chinese Academy of Medical Sciences & Peking Union Medical College · CN

Funding

Fundamental Research Funds for the Central public welfare research institutes ZXKT21007Fundamental Research Funds for the Central public welfare research institutes ZXKT23019Fundamental Research Funds for the Central public welfare research institutes ZZ13-YQ-046National Natural Science Foundation of China 81974550Scientific and Technological Innovation project of China Academy of Chinese Medical Science CI2021A04612Scientific and Technological Innovation project of China Academy of Chinese Medical Science CI2021B017-03
6 · The paper itself

Abstract

As a famous prescription in China, AnGong NiuHuang (AGNH) pill exerts good neuroprotection for ischaemic stroke (IS), but its mechanism is still unclear. In this study, the neuroprotection of AGNH was evaluated in the rat IS model which were established with the surgery of middle cerebral artery occlusion (MCAO), and the potential mechanism was elucidated by transcriptomic analysis and metabolomic analysis. AGNH treatment obviously decreased the infarct volume and Zea-Longa 5-point neurological deficit scores, improved the survival percentage of rats, regional cerebral blood flow (rCBF), and rat activity distance and activity time. Transcriptomics showed that AGNH exerted its anti-inflammatory effects by affecting the regulatory network including Tyrobp, Syk, Tlr2, Myd88 and Ccl2 as the core. Integrating transcriptomics and metabolomics identified 8 key metabolites regulated by AGNH, including L-histidine, L-serine, L-alanine, fumaric acid, malic acid, and N-(L-arginino) succinate, 1-pyrroline-4-hydroxy-2-carboxylate and 1-methylhistamine in the rats with IS. Additionally, AGNH obviously reduced Tyrobp, Syk, Tlr2, Myd88 and Ccl2 at both the mRNA and protein levels, decreased IL-1β, KC-GRO, IL-13, TNF-α, cleaved caspase 3 and p65 nucleus translocation, but increased IκBα expression. Network pharmacology analysis showed that quercetin, beta-sitosterol, baicalein, naringenin, acacetin, berberine and palmatine may play an important role in protecting against IS. Taken together, this study reveals that AGNH reduced neuroinflammation and protected against IS by inhibiting Tyrobp/Syk and Tlr2/Myd88, as well as NF-κB signalling pathway and regulating multiple metabolites.

Indexed as

Drugs, Chinese HerbalMetabolomicsRats, Sprague-DawleyTranscriptomeAnimalsGene Expression ProfilingInfarction, Middle Cerebral ArteryIschemic StrokeMaleMyeloid Differentiation Factor 88NeuroprotectionNeuroprotective AgentsRatsSignal TransductionDrugs, Chinese HerbalMyeloid Differentiation Factor 88Neuroprotective AgentsCerebral ischaemiaInflammationMyd88SykTlr2Tyrobp

Identifiers

PMID38401045
OpenAlexW4392131654

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.