Evidence map›Paper›PMID 38405663›Full record

ArticleBiochemistry and biophysics reports2024

Comparison of the efficiency of ultrafiltration, precipitation, and ultracentrifugation methods for exosome isolation.

Farshid Jaberi Ansari, Hossein Ahmadi Tafti, Amir Amanzadeh, Shahram Rabbani, Mohammad Ali Shokrgozar, Reza Heidari, Javad Behroozi, Hossein Eyni, Vladimir N Uversky, Hossein Ghanbari

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry and biophysics reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.

0numbers the graph read from it
0cells of the map it votes in
72citing papers in PubMed
17.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

72 citing papers in PubMed, 74 citations in OpenAlex.

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  18. Novel Liquid Biopsy in Gastrointestinal Cancers.Journal of gastroenterology and hepatology · 2026
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12 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Farshid Jaberi AnsariDepartment of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Hossein Ahmadi TaftiResearch Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Disease Research Institute, Tehran Heart Center Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Amir AmanzadehNational Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Shahram RabbaniResearch Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Disease Research Institute, Tehran Heart Center Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Ali ShokrgozarNational Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Reza HeidariMedical Biotechnology Research Center, AJA University of Medical Sciences, Tehran, 1411718541, Iran.
Javad BehrooziResearch Center for Cancer Screening and Epidemiology, AJA University of Medical Sciences, Tehran, Iran.
Hossein EyniStem Cell and Regenerative Medicine Research Center, Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Vladimir N UverskyDepartment of Molecular Medicine and USF Health Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, 33612, USA.
Hossein GhanbariDepartment of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Tehran University of Medical Sciences · IRAja University of Medical Sciences · IRPasteur Institute of Iran · IRIran University of Medical Sciences · IRUniversity of South Florida · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicles (EVs) are enclosed by a lipid-bilayer membrane and secreted by all types of cells. They are classified into three groups: apoptotic bodies, microvesicles, and exosomes. Exosomes play a number of important roles in the intercellular communication and crosstalk between tissues in the body. In this study, we use three common methods based on different principles for exosome isolation, namely ultrafiltration, precipitation, and ultracentrifugation. We use field emission scanning electron microscopy (FESEM) and dynamic light scattering (DLS) analyses for characterization of exosomes. The functionality and effect of isolated exosomes on the viability of hypoxic cells was investigated by alamarBlue and Flow-cytometry. The results of the FESEM study show that the ultrafiltration method isolates vesicles with higher variability of shapes and sizes when compared to the precipitation and ultracentrifugation methods. DLS results show that mean size of exosomes isolated by ultrafiltration, precipitation, and ultracentrifugation methods are 122, 89, and 60 nm respectively. AlamarBlue analysis show that isolated exosomes increase the viability of damaged cells by 11%, 15%, and 22%, respectively. Flow-cytometry analysis of damaged cells also show that these vesicles increase the content of live cells by 9%, 15%, and 20%, respectively. This study shows that exosomes isolated by the ultracentrifugation method are characterized by smaller size and narrow size distribution. Furthermore, more homogenous particles isolated by this method show increased efficiency of the protection of hypoxic cells in comparison with the exosomes isolated by the two other methods.

Indexed as

ExosomesIsolation methodsPrecipitationUltracentrifugationUltrafiltration

Identifiers

PMID38405663
PMCPMC10885727
OpenAlexW4392010334

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.