Evidence map›Paper›PMID 38407782›Full record

ArticleApplied biochemistry and biotechnology2024

High-Level Expression and Biochemical Characterization of a Maltotetraose Amylase in Pichia pastoris X-33 for Maltotetraose Production.

Xinyu Wang, Ming Xu, Xiaopeng Ren, Mingyu Li, Conggang Wang, Fan Yang, Xianzhen Li

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.2field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Xinyu WangSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Ming XuSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Xiaopeng RenSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Mingyu LiSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Conggang WangSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China. wangcg@dlpu.edu.cn.
Fan YangSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China. yang_fan@dlpu.edu.cn.
Xianzhen LiSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Dalian Polytechnic University · CN

Funding

Scientific Research Fund of Liaoning Provincial Education Department LJKFZ20220213
6 · The paper itself

Abstract

Maltotetraose amylase, which catalyzes the hydrolysis of amylaceous polysaccharides into maltooligosaccharides with maltotetraose as the main product, is extensively used in the food industry. However, the lack of efficient expression system for maltotetraose amylase has hampered its production and application. In this study, high-level production of a maltotetraose amylase mutant (referred to as Pp-Mta∆CBM) from Pseudomonas saccharophila was achieved in Pichia pastoris X-33. First, the gene of maltotetraose amylase with the carbohydrate-binding module (CBM) removed was codon-optimized and cloned into the pPICZαA vector, followed by transformation into P. pastoris X-33 for expression. Using the promoter P

Indexed as

AmylasesHydrogen-Ion ConcentrationMaltoseOligosaccharidesPichiaRecombinant ProteinsSaccharomycetalesAmylasesMaltosemaltotetraoseOligosaccharidesRecombinant ProteinsBiotransformation reactionCharacterizationHeterologous expressionMaltotetraose amylasePichia pastoris

Identifiers

PMID38407782
OpenAlexW4392164301

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.