Evidence map›Paper›PMID 38410796›Full record

ArticlePeerJ2024

Drop it all: extraction-free detection of targeted marine species through optimized direct droplet digital PCR.

Michelle Scriver, Ulla von Ammon, Cody Youngbull, Xavier Pochon, Jo-Ann L Stanton, Neil J Gemmell, Anastasija Zaiko

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Research progress on direct PCR and its applications.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2026
    Review
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Michelle ScriverBiosecurity Group, Cawthron Institute, Nelson, New Zealand.
Ulla von AmmonBiosecurity Group, Cawthron Institute, Nelson, New Zealand.
Cody YoungbullNucleic Sensing Systems, LCC, Saint Paul, Minnesota, United States.
Xavier PochonBiosecurity Group, Cawthron Institute, Nelson, New Zealand.
Jo-Ann L StantonDepartment of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.
Neil J GemmellDepartment of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand.ORCID 0000-0003-0671-3637
Anastasija ZaikoBiosecurity Group, Cawthron Institute, Nelson, New Zealand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Molecular biomonitoring programs increasingly use environmental DNA (eDNA) for detecting targeted species such as marine non-indigenous species (NIS) or endangered species. However, the current molecular detection workflow is cumbersome and time-demanding, and thereby can hinder management efforts and restrict the "opportunity window" for rapid management responses. Here, we describe a direct droplet digital PCR (direct-ddPCR) approach to detect species-specific free-floating extra-cellular eDNA (free-eDNA) signals,

Indexed as

BryozoaUrochordataAnimalsBiological MonitoringPolymerase Chain ReactionSeawaterBiomonitoringDirect-ddPCRDirect-PCRDroplet digital PCR (ddPCR)Environmental DNA (eDNA)Marine biosurveillanceMarine non-indigenous speciesMethod developmentSpecies-specific detection

Identifiers

PMID38410796
PMCPMC10896080

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.