Evidence map›Paper›PMID 38420846›Full record

ArticleJournal of the American Heart Association2024

Myosin Light Chain Phosphatase Plays an Important Role in Cardiac Fibrosis in a Model of Mineralocorticoid Receptor-Associated Hypertension.

Zhe Ye, Ryuji Okamoto, Hiromasa Ito, Rie Ito, Keishi Moriwaki, Mizuki Ichikawa, Lupiya Kimena, Yusuf Ali, Masaaki Ito, Celso E Gomez-Sanchez and 1 more

Open access · goldAbstract read
In one paragraph

Article in Journal of the American Heart Association, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Zhe YeDepartment of Cardiology and Nephrology Mie University Graduate School of Medicine Tsu Mie Japan.
Ryuji OkamotoDepartment of Cardiology and Nephrology Mie University Graduate School of Medicine Tsu Mie Japan.ORCID 0000-0002-8067-398X
Hiromasa ItoDepartment of Cardiology and Nephrology Mie University Graduate School of Medicine Tsu Mie Japan.
Rie ItoDepartment of Cardiology and Nephrology Mie University Graduate School of Medicine Tsu Mie Japan.
Keishi MoriwakiDepartment of Cardiology and Nephrology Mie University Graduate School of Medicine Tsu Mie Japan.ORCID 0000-0001-5385-7700
Mizuki IchikawaDepartment of Cardiology and Nephrology Mie University Graduate School of Medicine Tsu Mie Japan.
Lupiya KimenaDepartment of Cardiology and Nephrology Mie University Graduate School of Medicine Tsu Mie Japan.ORCID 0009-0007-9460-3847
Yusuf AliDepartment of Pharmacology and Toxicology University of Mississippi Medical Center Jackson MS.ORCID 0000-0003-0753-3590
Masaaki ItoDepartment of Cardiology and Nephrology Mie University Graduate School of Medicine Tsu Mie Japan.ORCID 0000-0002-1661-9647
Celso E Gomez-SanchezDepartment of Pharmacology and Toxicology University of Mississippi Medical Center Jackson MS.ORCID 0000-0002-9882-2082
Kaoru DohiDepartment of Cardiology and Nephrology Mie University Graduate School of Medicine Tsu Mie Japan.ORCID 0000-0002-5078-6326
Mie University · JPUniversity of Mississippi Medical Center · US

Funding

Adrenal cell ATP1A1 mutations and mechanisms of aldosterone biosynthesisR01HL144847 · NHLBI · UNIVERSITY OF MISSISSIPPI MED CTR · PI GOMEZ-SANCHEZ, CELSO ENRIQUE · 2019 to 2022
$1.3M
Regulation of Mineralocorticoid Receptor ActionI01BX004681 · VA · G V SONNY MONTGOMERY VA MEDCIAL CENTER · PI GOMEZ-SANCHEZ, CELSO ENRIQUE · 2021 to 2023
–
BLRD VA I01 BX004681NHLBI NIH HHS R01 HL144847
6 · The paper itself

Abstract

backgroundMyosin phosphatase targeting subunit 2 (MYPT2) is an important subunit of cardiac MLC (myosin light chain) phosphatase, which plays a crucial role in regulating the phosphorylation of MLC to phospho-MLC (p-MLC). A recent study demonstrated mineralocorticoid receptor-related hypertension is associated with RhoA/Rho-associated kinase/MYPT1 signaling upregulation in smooth muscle cells. Our purpose is to investigate the effect of MYPT2 on cardiac function and fibrosis in mineralocorticoid receptor-related hypertension. METHODS AND

resultsHL-1 murine cardiomyocytes were incubated with different concentrations or durations of aldosterone. After 24-hour stimulation, aldosterone increased CTGF (connective tissue growth factor) and MYPT2 and decreased p-MLC in a dose-dependent manner. MYPT2 knockdown decreased CTGF. Cardiac-specific MYPT2-knockout (c-MYPT2

conclusionsCardiac-specific MYPT2 knockout leads to decreased myosin light chain phosphatase and increased p-MLC. MYPT2 deletion prevented cardiac fibrosis and dysfunction in a model of mineralocorticoid receptor-associated hypertension.

Indexed as

HypertensionMyosin-Light-Chain PhosphataseAldosteroneAnimalsFibrosisMiceMyocytes, CardiacPhosphorylationReceptors, MineralocorticoidAldosteroneMyosin-Light-Chain PhosphatasePpp1r12a protein, mouseReceptors, Mineralocorticoidaldosteronecardiac fibrosismineralocorticoid receptormyosin light chainphosphorylation

Identifiers

PMID38420846
PMCPMC10944028
OpenAlexW4392293557

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.