Evidence map›Paper›PMID 38422111›Full record

ArticlePloS one2024

Microindentation of fresh soft biological tissue: A rapid tissue sectioning and mounting protocol.

Clíona M McCarthy, Kevin L McKevitt, Sinéad A Connolly, Isabel Andersson, Fiona C Leahy, Siobhan Egan, Michael A Moloney, Eamon G Kavanagh, Colin Peirce, Eoghan M Cunnane and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Clíona M McCarthyBiomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.
Kevin L McKevittDepartment of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland.
Sinéad A ConnollyBiomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.ORCID 0000-0002-8953-6105
Isabel AnderssonBiomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.
Fiona C LeahyDepartment of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland.
Siobhan EganDepartment of Colorectal Surgery, University Hospital Limerick, Limerick, Ireland.
Michael A MoloneyDepartment of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland.
Eamon G KavanaghDepartment of Vascular & Endovascular Surgery, University Hospital Limerick, Limerick, Ireland.
Colin PeirceDepartment of Colorectal Surgery, University Hospital Limerick, Limerick, Ireland.
Eoghan M CunnaneBiomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.
Kieran D McGourtyBiomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.
Michael T WalshBiomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.
John J E MulvihillBiomaterials Cluster, Bernal Institute, University of Limerick, Limerick, Ireland.ORCID 0000-0003-0437-0351

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microindentation of fresh biological tissues is necessary for the creation of 3D biomimetic models that accurately represent the native extracellular matrix microenvironment. However, tissue must first be precisely sectioned into slices. Challenges exist in the preparation of fresh tissue slices, as they can tear easily and must be processed rapidly in order to mitigate tissue degradation. In this study, we propose an optimised mounting condition for microindentation and demonstrate that embedding tissue in a mixture of 2.5% agarose and 1.5% gelatin is the most favourable method of tissue slice mounting for microindentation. This protocol allows for rapid processing of fresh biological tissue and is applicable to a variety of tissue types.

Indexed as

BiomimeticsExtracellular MatrixFoodGelatinHistocompatibility TestingGelatin

Identifiers

PMID38422111
PMCPMC10903917

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.