Evidence map›Paper›PMID 38431671›Full record

ArticleNature communications2024

High-density volumetric super-resolution microscopy.

Sam Daly, João Ferreira Fernandes, Ezra Bruggeman, Anoushka Handa, Ruby Peters, Sarah Benaissa, Boya Zhang, Joseph S Beckwith, Edward W Sanders, Ruth R Sims and 4 more

Abstract read
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
  2. Virtual Overlay Staining With Plasmonic Oligomer Metasurface.Small (Weinheim an der Bergstrasse, Germany) · 2026
    Article
  3. Article
  4. Volumetric Single-Molecule Tracking Inside Subcellular Structures.Small (Weinheim an der Bergstrasse, Germany) · 2026
    Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Sam DalyYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.ORCID 0000-0002-8559-3161
João Ferreira FernandesRadcliffe Department of Medicine and MRC Human Immunology Unit, John Radcliffe Hospital, University of Oxford, Oxford, OX3 9DU, UK.ORCID 0000-0002-8161-6709
Ezra BruggemanYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.ORCID 0000-0001-6100-1443
Anoushka HandaYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
Ruby PetersDepartment of Physiology, Development, and Neuroscience, University of Cambridge, Cambridge, CB2 3EL, UK.
Sarah BenaissaCambridge Advanced Imaging Centre, Downing Site, University of Cambridge, Cambridge, CB2 3DY, UK.
Boya ZhangCambridge Advanced Imaging Centre, Downing Site, University of Cambridge, Cambridge, CB2 3DY, UK.
Joseph S BeckwithYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.ORCID 0000-0003-4726-230X
Edward W SandersYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.ORCID 0000-0002-8972-7702
Ruth R SimsWavefront-Engineering Microscopy Group, Photonics Department, Institut de la Vision, Sorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France.ORCID 0000-0001-9864-6405
David KlenermanYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.ORCID 0000-0001-7116-6954
Simon J DavisRadcliffe Department of Medicine and MRC Human Immunology Unit, John Radcliffe Hospital, University of Oxford, Oxford, OX3 9DU, UK.
Kevin O'HolleranCambridge Advanced Imaging Centre, Downing Site, University of Cambridge, Cambridge, CB2 3DY, UK.
Steven F LeeYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK. sl591@cam.ac.uk.ORCID 0000-0003-4492-5139

Funding

Royal Society RGF\EA\181021
6 · The paper itself

Abstract

Volumetric super-resolution microscopy typically encodes the 3D position of single-molecule fluorescence into a 2D image by changing the shape of the point spread function (PSF) as a function of depth. However, the resulting large and complex PSF spatial footprints reduce biological throughput and applicability by requiring lower labeling densities to avoid overlapping fluorescent signals. We quantitatively compare the density dependence of single-molecule light field microscopy (SMLFM) to other 3D PSFs (astigmatism, double helix and tetrapod) showing that SMLFM enables an order-of-magnitude speed improvement compared to the double helix PSF by resolving overlapping emitters through parallax. We demonstrate this optical robustness experimentally with high accuracy ( > 99.2 ± 0.1%, 0.1 locs μm

Indexed as

Imaging, Three-DimensionalMicroscopyNanotechnologySingle Molecule Imaging

Identifiers

PMID38431671
PMCPMC10908787

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.