Evidence map›Paper›PMID 38448814›Full record

ArticleBMC genomics2024

Integrated analysis reveals the regulatory mechanism of the neddylation inhibitor MLN4924 on the metabolic dysregulation in rabbit granulosa cells.

Mengjuan Chen, Yuqing Liu, Mingzhong Zuo, Meina Zhang, Zhitong Wang, Xin Li, Dongdong Yuan, Huifen Xu, Guangqing Yu, Ming Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 5 citations in OpenAlex.

  1. Role of neddylation in diabetes metabolism: potential therapeutic target.Journal of diabetes and metabolic disorders · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Mengjuan ChenCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Yuqing LiuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Mingzhong ZuoCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Meina ZhangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Zhitong WangCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Xin LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Dongdong YuanCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Huifen XuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China.
Guangqing YuCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China. ygq@henau.edu.cn.
Ming LiCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, P. R. China. 13803849306@163.com.
Henan Agricultural University · CN

Funding

National Key Research and Development Program of China 2018YFD0502203National Natural Science Foundation of China Grant 32102833Special Fund for Henan Agriculture Research System HARS-22-13-G1
6 · The paper itself

Abstract

backgroundNeddylation, an important post-translational modification (PTM) of proteins, plays a crucial role in follicular development. MLN4924 is a small-molecule inhibitor of the neddylation-activating enzyme (NAE) that regulates various biological processes. However, the regulatory mechanisms of neddylation in rabbit ovarian cells have not been emphasized. Here, the transcriptome and metabolome profiles in granulosa cells (GCs) treated with MLN4924 were utilized to identify differentially expressed genes, followed by pathway analysis to precisely define the altered metabolisms.

resultsThe results showed that 563 upregulated and 910 downregulated differentially expressed genes (DEGs) were mainly enriched in pathways related to cancer, cell cycle, PI3K-AKT, progesterone-mediated oocyte maturation, and PPAR signaling pathway. Furthermore, we characterized that MLN4924 inhibits PPAR-mediated lipid metabolism, and disrupts the cell cycle by promoting the apoptosis and proliferation of GCs. Importantly, we found the reduction of several metabolites in the MLN4924 treated GCs, including glycerophosphocholine, arachidic acid, and palmitic acid, which was consistent with the deregulation of PPAR signaling pathways. Furthermore, the increased metabolites included 6-Deoxy-6-sulfo-D-glucono-1,5-lactone and N-Acetyl-D-glucosaminyldiphosphodolichol. Combined with transcriptome data analyses, we identified genes that strongly correlate with metabolic dysregulation, particularly those related to glucose and lipid metabolism. Therefore, neddylation inhibition may disrupt the energy metabolism of GCs.

conclusionsThese results provide a foundation for in-depth research into the role and molecular mechanism of neddylation in ovary development.

Indexed as

CyclopentanesPeroxisome Proliferator-Activated ReceptorsPhosphatidylinositol 3-KinasesPyrimidinesAnimalsFemaleGranulosa CellsLipid MetabolismRabbitsCyclopentanesPeroxisome Proliferator-Activated ReceptorspevonedistatPhosphatidylinositol 3-KinasesPyrimidinesGranulosa cellsMetabolomeMLN4924NeddylationRabbitTranscriptome

Identifiers

PMID38448814
PMCPMC10916191
OpenAlexW4392509507

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.