Evidence map›Paper›PMID 38463588›Full record

ArticleAmerican journal of translational research2024

Dysregulation of kidney proteases in the pathogenesis of hypertension following unilateral nephrectomy in juvenile mice.

Rasha Aly, Yunus E Dogan, Niharika Bala, Carlos Lugo, Seena Darwish, Lawrence Shoemaker, Abdel A Alli

Open access · bronzeAbstract read
PubMed Full text at PMC
In one paragraph

Article in American journal of translational research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Rasha AlyDepartment of Pediatrics, Division of Pediatric Nephrology, University of Florida College of Medicine Gainesville, FL, USA.
Yunus E DoganDepartment of Physiology and Aging, University of Florida College of Medicine Gainesville, FL, USA.
Niharika BalaDepartment of Physiology and Aging, University of Florida College of Medicine Gainesville, FL, USA.
Carlos LugoDepartment of Physiology and Aging, University of Florida College of Medicine Gainesville, FL, USA.
Seena DarwishDepartment of Physiology and Aging, University of Florida College of Medicine Gainesville, FL, USA.
Lawrence ShoemakerDepartment of Pediatrics, Division of Pediatric Nephrology, University of Florida College of Medicine Gainesville, FL, USA.
Abdel A AlliDepartment of Pediatrics, Division of Pediatric Nephrology, University of Florida College of Medicine Gainesville, FL, USA.
University of Florida · US

Funding

The circadian clock protein BMAL and post-translational regulation of ENaC in the kidneyR01DK123078 · NIDDK · UNIVERSITY OF FLORIDA · PI ALLI, ABDEL AYUBE · 2020 to 2024
$1.7M
NIDDK NIH HHS R01 DK123078
6 · The paper itself

Abstract

backgroundUnliteral nephrectomy (UNX) results in the reduction of kidney mass. The remaining kidney undergoes compensatory renal growth via hypertrophy of the glomeruli and renal tubules to maintain a normal glomerular filtration rate (GFR). These compensatory mechanisms result in increased capillary pressure and glomerular hyperfiltration to increase single nephron GFR. Over time, hyperfiltration may lead to kidney scarring and the development of hypertension.

objectivesThe first objective of this study was to test the hypothesis that a 50% reduction in functioning nephrons in juvenile mice leads to increased blood pressure over a 24-hour phase. The second objective was to test the hypothesis that UNX leads to changes in the expression and activity of kidney proteases in juvenile mice.

methodsEight male C57B6 juvenile wild-type mice were subject to UNX and an equal number of mice were subject to sham (SH) surgery. Metabolic cage studies were performed for 5 weeks to collect urine produced during the inactive and active phases. Blood pressure was measured using the tail cuff method twice weekly and tail blood was collected on different days during the inactive or active phase of each animal. The mice were euthanized at the age of 9 weeks. Western blotting and immunohistochemistry were performed to investigate changes in renal protein expression of various cathepsins and renal kallikrein 1 (KLK1) between the two groups. Protease activity assays were performed using kidney lysates and urine samples from each group.

resultsCompared to the SH group, UNX mice showed a persistent increase in blood pressure at week 3 which progressed toward the end of the study at week 5 of age. Cathepsin B, D, and S expression and activity were up-regulated in kidney cortex lysates from UNX mice compared to the SH control group. KLK1 protein expression was down-regulated and urinary nitric oxide excretion was decreased in UNX mice compared to the SH control group.

conclusionUNX results in the development of persistent and progressive hypertension. Down-regulation of KLK1 and up-regulation of various cathepsins may contribute to the development of hypertension via multiple mechanisms including a decrease in nitric oxide (NO) production.

Indexed as

cathepsinshypertensionkidney proteasesrenal kallikreinUnilateral nephrectomy

Identifiers

PMID38463588
PMCPMC10918144
OpenAlexW4393045162

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.