Evidence map›Paper›PMID 38468104›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Multimodal Control of Bacterial Gene Expression by Red and Blue Light.

Stefanie S M Meier, Elina Multamäki, Américo T Ranzani, Heikki Takala, Andreas Möglich

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Stefanie S M MeierDepartment of Biochemistry, University of Bayreuth, Bayreuth, Germany.ORCID 0009-0002-6028-5223
Elina MultamäkiDepartment of Anatomy, University of Helsinki, Helsinki, Finland.ORCID 0000-0002-0636-9269
Américo T RanzaniDepartment of Biochemistry, University of Bayreuth, Bayreuth, Germany.ORCID 0000-0002-6203-9467
Heikki TakalaDepartment of Anatomy, University of Helsinki, Helsinki, Finland.ORCID 0000-0003-2518-8583
Andreas MöglichDepartment of Biochemistry, University of Bayreuth, Bayreuth, Germany. andreas.moeglich@uni-bayreuth.de.ORCID 0000-0002-7382-2772

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable innovative use cases in cell biology, synthetic biology, and biotechnology. In this chapter, we detail the implementation of the pREDusk, pREDawn, pCrepusculo, and pAurora optogenetic circuits for controlling bacterial gene expression by red and blue light, respectively. The protocols provided here guide the practical use and multiplexing of these circuits, thereby enabling graded protein production in bacteria at analytical and semi-preparative scales.

Indexed as

BacteriaBlue LightGene ExpressionLightOptogeneticsANTARBacteriophytochromeGene expressionHistidine kinaseLight-oxygen-voltageOptogeneticsRNA bindingSensory photoreceptorSynthetic biologyTwo-component system

Identifiers

PMID38468104

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.