ArticleBMC veterinary research2024
Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology.
Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Thiram inhibits human sperm motility and capacitation by increasing mitochondrial OTranslational andrology and urology · 2026Article
- Synergistic amelioration of heat stress-induced testicular damage and impaired fertility by sesame seed oil and α-lipoic acid in male rats.Basic and clinical andrology · 2026Article
- Comparing the efficacy of dextrose, mannitol, and saline solutions in male rabbits' chemical castration.Animal models and experimental medicine · 2026Article
- Moringa oleifera Lam. As a Herbal Antioxidant-Enriched Semen Extender in Cryopreservation: A Critical Review of Its Protective Potential.Veterinary medicine and science · 2026Review
- Can monomethyl fumarate and nifedipine reduce ischemia reperfusion injury in rat ovary, and what is the share of oxidative stress-sensitive transcription factors HIF-1α, NF-κB, and Nrf2?Iranian journal of basic medical sciences · 2026Article
- Efficacy of zinc oxide nanoparticles in chemical castration of male Wistar rats.Scientific reports · 2025Article
- Intratesticular copper oxide nanoparticles induce dose-dependent chemical castration in male mice.Scientific reports · 2025Article
- Oral niacin mitigates heat-induced reproductive impairments in male mice.Scientific reports · 2025Article
- β-Glucan and resveratrol mitigate lead induced reproductive toxicity in male mice.Scientific reports · 2025Article
- The synergistic activity of fisetin on quercetin improves testicular recover in ischemia-reperfusion injury in rats.Scientific reports · 2025Article
- Effect of Inhibin Immunization on Reproductive Hormones and Testicular Morphology of Dezhou Donkeys During the Non-Breeding Season.Animals : an open access journal from MDPI · 2025Article
- Possible role of vitamins C and E co-administration in the prevention of testicular ischemia-reperfusion injury following surgical repair of torsion of the testis.Frontiers in nutrition · 2025Review
- Evaluation of chemical castration by intra-testicular injection of zinc-doped carbon dots in mature rats.Veterinary research forum : an international quarterly journal · 2025Article
- Male animal sterilization: history, current practices, and potential methods for replacing castration.Frontiers in veterinary science · 2024Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe aim of the present study was to examine donkey sperm quality after intratesticular injection of hypertonic mannitol (HM) and saline (HS).
methodsRandomly assigned to five treatment groups were 15 adult male donkeys: (1) Control group (no treatment), (2) Surgery group (surgical castration for testosterone control), (3) NS group (normal saline intratesticular injection), (4) HS group (hypertonic saline), and (5) HM group. We injected 20 mL per testicle. We took 5 mL blood from all donkeys before injection. Castration was performed under general anesthesia 60 days later. Samples included blood and testicular tissue. Total motility (TM), progressive motility (PM), movementy features, DNA damage, morphology, viability, and plasma membrane functionality were evaluated. Hormone analyses, histomorphometric studies and oxidative stress indices including total antioxidant capacity (TAC), glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), and NADP+/NADPH were evaluated. Apoptosis, pyroptosis-related Bax, Caspase-1, GSDMD, and Bcl-2 expression were also assessed.
resultsIn HS and HM groups, testosterone, epididymal sperm count, motility, viability, and plasma membrane functionality dropped while sperm DNA damage increased. HS and HM groups had significantly lower histomorphometric parameters, TAC, GPx, SOD, GSH, and Bcl-2 gene expression. MDA, NADP
conclusionsToxic effects of hypertonic saline and mannitol on reproductive parameters were seen following, hence, they might be considered as a good chemical sterilizing treatment in donkeys.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.