Evidence map›Paper›PMID 38478299›Full record

ArticleProbiotics and antimicrobial proteins2025

Novel Probiotic Candidates in Artisanal Feta-Type Kefalonian Cheese: Unveiling a Still-Undisclosed Biodiversity.

Iliada K Lappa, Aikaterini Natsia, Dimitra Alimpoumpa, Electra Stylianopoulou, Ioanna Prapa, Konstantinos Tegopoulos, Chrysoula Pavlatou, George Skavdis, Aikaterini Papadaki, Nikolaos Kopsahelis

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Probiotics and antimicrobial proteins, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Iliada K LappaDepartment of Food Science and Technology, Ionian University, 28100, Argostoli, Kefalonia, Greece.
Aikaterini NatsiaDepartment of Food Science and Technology, Ionian University, 28100, Argostoli, Kefalonia, Greece.
Dimitra AlimpoumpaDepartment of Food Science and Technology, Ionian University, 28100, Argostoli, Kefalonia, Greece.
Electra StylianopoulouDepartment of Molecular Biology & Genetics, Democritus University of Thrace, 68100, Alexandroupolis, Greece.
Ioanna PrapaDepartment of Molecular Biology & Genetics, Democritus University of Thrace, 68100, Alexandroupolis, Greece.
Konstantinos TegopoulosDepartment of Molecular Biology & Genetics, Democritus University of Thrace, 68100, Alexandroupolis, Greece.
Chrysoula PavlatouDepartment of Molecular Biology & Genetics, Democritus University of Thrace, 68100, Alexandroupolis, Greece.
George SkavdisDepartment of Molecular Biology & Genetics, Democritus University of Thrace, 68100, Alexandroupolis, Greece.
Aikaterini PapadakiDepartment of Food Science and Technology, Ionian University, 28100, Argostoli, Kefalonia, Greece.
Nikolaos KopsahelisDepartment of Food Science and Technology, Ionian University, 28100, Argostoli, Kefalonia, Greece. kopsahelis@ionio.gr.
Democritus University of Thrace · GRIonian University · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Autochthonous dairy lactic acid bacteria (LAB) isolates encompass a natural source of starter, adjunct, or probiotic candidates. In this context, traditionally manufactured, using exclusively animal rennet, Feta-type cheeses were collected from five farms located in different regions of Kefalonia island (Greece). The primary objective of this study was to isolate and characterize novel LAB, thereby exploring the unmapped microbial communities of Kefalonian Feta-type cheese and identifying new potential probiotics. The initial screening, included a preliminary gastrointestinal (GI) tolerance assessment (acidic conditions and bile salts), followed by their safety evaluation (hemolytic activity and antibiotic susceptibility). Based on the preliminary screening, selected strains underwent molecular identification and were further investigated for their probiotic attributes (lysozyme and phenol resistance, antimicrobial traits, antidiabetic aspects, cholesterol reduction and adhesion, adhesion to Caco-2 cells, and milk acidification potential). The results showed that 49, out of the 93 retrieved isolates, exhibited resistance to GI conditions, whereas 18 met the safety criteria. The molecular identification revealed strains belonging to the species Lactiplantibacillus plantarum, Limosilactobacillus fermentum, Lacticaseibacillus rhamnosus, and Lacticaseibacillus paracasei. The selected rod-shaped 14 isolates displayed a potential probiotic character. The best-performing isolates concerning cholesterol assimilation and adhesion, α-glucosidase inhibition, and epithelial adherence were Lpb. plantarum F89, F162, and F254 and Lcb. paracasei F214 and F216, whereas Lcb. paracasei F70 showed potential as a defined strain starter. The present study explores for the first time the biodiversity of traditionally fermented microbial communities in Kefalonian Feta-type cheese, revealing novel potential probiotic strains that can contribute to the development of innovative functional food products.

Indexed as

CheeseLactobacillalesProbioticsAnimalsBiodiversityCaco-2 CellsFood MicrobiologyGreeceHumansAutochthonous isolatesFunctionalLactic acid bacteriaProbiotic propertiesSustainable food systemsWhite brine cheese

Identifiers

PMID38478299
PMCPMC12532650
OpenAlexW4392762468

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.