Evidence map›Paper›PMID 38494699›Full record

ArticleThe Journal of veterinary medical science2024

Performance evaluation of a rapid immunochromatographic test kit in detecting bovine mastitis-causing streptococci.

Yusaku Tsugami, Yuya Nagasawa, Aoi Sugiyama, Yu Ando, Kotaro Oda, Koji Maehana, Tetsu Obayashi, Tomohito Hayashi

Open access · diamondAbstract readEvaluation Study
In one paragraph

Article in The Journal of veterinary medical science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.3field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Yusaku TsugamiPathology and Production Disease Group, Division of Hygiene Management, National Institute of Animal Health, National Agriculture and Food Research Organization, Hokkaido, Japan.
Yuya NagasawaPathology and Production Disease Group, Division of Hygiene Management, National Institute of Animal Health, National Agriculture and Food Research Organization, Hokkaido, Japan.
Aoi SugiyamaPathology and Production Disease Group, Division of Hygiene Management, National Institute of Animal Health, National Agriculture and Food Research Organization, Hokkaido, Japan.
Yu AndoResearch Laboratory of Advanced Science & Technology, Corporate Research & Development, Asahi Kasei Corporation, Shizuoka, Japan.
Kotaro OdaResearch Laboratory of Advanced Science & Technology, Corporate Research & Development, Asahi Kasei Corporation, Shizuoka, Japan.
Koji MaehanaResearch Laboratory of Advanced Science & Technology, Corporate Research & Development, Asahi Kasei Corporation, Shizuoka, Japan.
Tetsu ObayashiShikaoi Veterinary Clinic, NOSAI Hokkaido, Hokkaido, Japan.
Tomohito HayashiPathology and Production Disease Group, Division of Hygiene Management, National Institute of Animal Health, National Agriculture and Food Research Organization, Hokkaido, Japan.
Asahi Kasei (Japan) · JPNational Institute of Animal Health · JPNational Agriculture and Food Research Organization · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mastitis causes significant economic losses to the dairy industry due to decreased milk production in infected cows. Identification of mastitis-causing pathogens, such as streptococci, is necessary for selecting an effective antibiotic for treating mastitis. Although bacterial cultivation is widely used for pathogen identification, it requires more than 24 hr to complete. Contrarily, Lateral flow assays are simple, rapid, and inexpensive testing procedures. In this study, the effectiveness of an immunochromatographic test kit for detecting streptococci in milk samples from cows with clinical mastitis was evaluated as an alternative to bacterial cultivation. The performance of the immunochromatographic test kit for detecting mastitis-causing pathogens was compared with that of bacterial cultivation and real-time quantitative polymerase chain reaction (qPCR). The sensitivity and specificity of the immunochromatographic test kit were 0.800 and 0.875, respectively, compared with bacterial cultivation. Additionally, the κ statistic values of the immunochromatographic test kit was 0.667, indicating substantial agreement with the results of bacterial cultivation. Statistically, sensitivity and specificity of the immunochromatographic kit and real-time qPCR did not differ significantly; thus, the immunochromatographic test kit detected mastitis-causing streptococci as effectively as real-time qPCR. Therefore, the immunochromatographic kit is a rapid, inexpensive, and simple method for detecting streptococci and contributes to the timely selection of appropriate antibiotics for treatment and promotes early recovery from mastitis.

Indexed as

Chromatography, AffinityMastitis, BovineMilkSensitivity and SpecificityStreptococcal InfectionsStreptococcusAnimalsCattleFemaleReagent Kits, DiagnosticReal-Time Polymerase Chain ReactionReagent Kits, Diagnosticbovine mastitisimmunochromatographymilkstreptococci

Identifiers

PMID38494699
PMCPMC11144529
OpenAlexW4392891890

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.