Evidence map›Paper›PMID 38503290›Full record

ArticleCell reports methods2024

Discontinuity third harmonic generation microscopy for label-free imaging and quantification of intraepidermal nerve fibers.

Pei-Jhe Wu, Hsiao-Chieh Tseng, Chi-Chao Chao, Yi-Hua Liao, Chen-Tung Yen, Wen-Ying Lin, Sung-Tsang Hsieh, Wei-Zen Sun, Chi-Kuang Sun

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Article in Cell reports methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Pei-Jhe WuDepartment of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Hsiao-Chieh TsengDepartment of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Chi-Chao ChaoDepartment of Neurology, National Taiwan University Hospital, and National Taiwan University College of Medicine, Taipei 100225, Taiwan.
Yi-Hua LiaoDepartment of Dermatology, National Taiwan University Hospital, and National Taiwan University College of Medicine Taipei 100225, Taiwan.
Chen-Tung YenDepartment of Life Science, National Taiwan University, Taipei 10617, Taiwan.
Wen-Ying LinDepartment of Life Science, National Taiwan University, Taipei 10617, Taiwan; Department of Anesthesiology, National Taiwan University Hospital, and National Taiwan University College of Medicine, Taipei 100225, Taiwan.
Sung-Tsang HsiehDepartment of Neurology, National Taiwan University Hospital, and National Taiwan University College of Medicine, Taipei 100225, Taiwan. Electronic address: shsieh@ntu.edu.tw.
Wei-Zen SunDepartment of Anesthesiology, National Taiwan University Hospital, and National Taiwan University College of Medicine, Taipei 100225, Taiwan. Electronic address: wzsun@ntu.edu.tw.
Chi-Kuang SunDepartment of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan; Graduate Institute of Biomedical Electronics and Bioinformatics and Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan. Electronic address: sun@ntu.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Label-free imaging methodologies for nerve fibers rely on spatial signal continuity to identify fibers and fail to image free intraepidermal nerve endings (FINEs). Here, we present an imaging methodology-called discontinuity third harmonic generation (THG) microscopy (dTHGM)-that detects three-dimensional discontinuities in THG signals as the contrast. We describe the mechanism and design of dTHGM and apply it to reveal the bead-string characteristics of unmyelinated FINEs. We confirmed the label-free capability of dTHGM through a comparison study with the PGP9.5 immunohistochemical staining slides and a longitudinal spared nerve injury study. An intraepidermal nerve fiber (IENF) index based on a discontinuous-dot-connecting algorithm was developed to facilitate clinical applications of dTHGM. A preliminary clinical study confirmed that the IENF index was highly correlated with skin-biopsy-based IENF density (Pearson's correlation coefficient R = 0.98) and could achieve differential identification of small-fiber neuropathy (p = 0.0102) in patients with diabetic peripheral neuropathy.

Indexed as

Diabetic NeuropathiesSecond Harmonic Generation MicroscopySmall Fiber NeuropathyHumansNerve FibersSkinCP: imagingCP: neurosciencediabetic peripheral neuropathyfree intraepidermal nerve endingsnoninvasive imaging techniqueoptical biopsyskin biopsyskin innervationsmall-fiber neuropathythird harmonic generationunmyelinated nerve fibers

Identifiers

PMID38503290
PMCPMC10985268

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.