Evidence mapPaperPMID 38529047Full record

ArticleJournal of diabetes research2024

Identification and Validation of the Pyroptosis-Related Hub Gene Signature and the Associated Regulation Axis in Diabetic Keratopathy.

Yi Cui, Li Wang, Wentao Liang, Li Huang, Shuting Zhuang, Hong Shi, Nuo Xu, Jianzhang Hu

Open access · goldAbstract read
In one paragraph

Article in Journal of diabetes research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Yi CuiDepartment of Ophthalmology, Fujian Medical University Union Hospital, Fuzhou, Fujian, China.ORCID https://orcid.org/0000-0002-0936-157X
Li WangDepartment of Ophthalmology, Fujian Medical University Union Hospital, Fuzhou, Fujian, China.
Wentao LiangDepartment of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27101, USA.ORCID https://orcid.org/0000-0002-0609-6280
Li HuangDepartment of Ophthalmology, Fujian Medical University Union Hospital, Fuzhou, Fujian, China.
Shuting ZhuangCollege of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China.ORCID https://orcid.org/0000-0001-9112-978X
Hong ShiCollege of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China.
Nuo XuDepartment of Ophthalmology, Fujian Provincial Hospital, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China.ORCID https://orcid.org/0000-0003-2833-9017
Jianzhang HuDepartment of Ophthalmology, Fujian Medical University Union Hospital, Fuzhou, Fujian, China.ORCID https://orcid.org/0000-0003-0610-7856
Fujian Medical University · CNFujian University of Traditional Chinese Medicine · CNWake Forest University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Diabetic keratopathy (DK) poses a significant challenge in diabetes mellitus, yet its molecular pathways and effective treatments remain elusive. The aim of our research was to explore the pyroptosis-related genes in the corneal epithelium of the streptozocin-induced diabetic rats. Methods: After sixteen weeks of streptozocin intraperitoneal injection, corneal epithelium from three diabetic rats and three normal groups underwent whole-transcriptome sequencing. An integrated bioinformatics pipeline, including differentially expressed gene (DEG) identification, enrichment analysis, protein-protein interaction (PPI) network, coexpression, drug prediction, and immune deconvolution analyses, identified hub genes and key drivers in DK pathogenesis. These hub genes were subsequently validated in vivo through RT-qPCR. Results: A total of 459 DEGs were screened out from the diabetic group and nondiabetic controls. Gene Set Enrichment Analysis highlighted significant enrichment of the NOD-like receptor, Toll-like receptor, and NF-kappa B signaling pathways. Intersection of DEGs and pyroptosis-related datasets showed 33 differentially expressed pyroptosis-related genes (DEPRGs) associated with pathways such as IL-17, NOD-like receptor, TNF, and Toll-like receptor signaling. A competing endogenous RNA network comprising 16 DEPRGs, 22 lncRNAs, 13 miRNAs, and 3 circRNAs was constructed. After PPI network, five hub genes ( Conclusions: The identified and validated hub genes,

Indexed as

Diabetes Mellitus, ExperimentalPyroptosisAnimalsComputational BiologyCyclooxygenase 2Interleukin-18RatsStreptozocinTNF Receptor-Associated Factor 6Cyclooxygenase 2Interleukin-18StreptozocinTNF Receptor-Associated Factor 6

Identifiers

PMID38529047
PMCPMC10963115
OpenAlexW4392931464

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.