ArticleNucleic acids research2024
A phage nucleus-associated RNA-binding protein is required for jumbo phage infection.
Article in Nucleic acids research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- The biology of jumbo phages.Nature communications · 2026Review
- The phage nucleus synergizes with an anti-defense protein to resist bacterial immunity.Cell reports · 2026Article
- Enzyme-enhanced RNA isolation from biofilm-producing bacteria.Microbiology spectrum · 2026Article
- Phage resistance bidirectionally altered antibiotic susceptibility in Klebsiella pneumoniae via galE mutation.International journal of antimicrobial agents · 2026Article
- Molecular basis for anti-jumbo phage immunity by AVAST type 5.Molecular cell · 2026Article
- Bacteriophage genome-wide transposon mutagenesis.bioRxiv : the preprint server for biology · 2025Article
- The complex developmental mechanisms of nucleus-forming jumbo phages.Current opinion in microbiology · 2025Review
- Unexplored diversity and molecular genetic signatures of chimallin and phuz encoding phages.BMC genomics · 2025Article
- The Biology of Nucleus-Forming Jumbo Phages.Annual review of genetics · 2025Review
- Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.Cell host & microbe · 2025Article
- CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d.Nature microbiology · 2025Article
- Article
- A ribosome-interacting jumbophage protein associates with the phage nucleus to facilitate efficient propagation.PLoS pathogens · 2025Article
- Genome-wide identification of bacterial genes contributing to nucleus-forming jumbo phage infection.Nucleic acids research · 2025Article
- Erwinia phage Asesino is a nucleus-forming phage that lacks PhuZ.Scientific reports · 2025Article
- A transcriptionally active lipid vesicle encloses the injectedbioRxiv : the preprint server for biology · 2024Article
- Exploring pangenomic diversity and CRISPR-Cas evasion potential in jumbo phages: a comparative genomics study.Microbiology spectrum · 2024Article
- Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection.Cell host & microbe · 2024Article
- The intricate organizational strategy of nucleus-forming phages.Current opinion in microbiology · 2024Review
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Authors and funding
21 authors at 4 institutions in 2 countries.
Funding
Abstract
Large-genome bacteriophages (jumbo phages) of the proposed family Chimalliviridae assemble a nucleus-like compartment bounded by a protein shell that protects the replicating phage genome from host-encoded restriction enzymes and DNA-targeting CRISPR-Cas nucleases. While the nuclear shell provides broad protection against host nucleases, it necessitates transport of mRNA out of the nucleus-like compartment for translation by host ribosomes, and transport of specific proteins into the nucleus-like compartment to support DNA replication and mRNA transcription. Here, we identify a conserved phage nuclear shell-associated protein that we term Chimallin C (ChmC), which adopts a nucleic acid-binding fold, binds RNA with high affinity in vitro, and binds phage mRNAs in infected cells. ChmC also forms phase-separated condensates with RNA in vitro. Targeted knockdown of ChmC using mRNA-targeting dCas13d results in accumulation of phage-encoded mRNAs in the phage nucleus, reduces phage protein production, and compromises virion assembly. Taken together, our data show that the conserved ChmC protein plays crucial roles in the viral life cycle, potentially by facilitating phage mRNA translocation through the nuclear shell to promote protein production and virion development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.