Evidence map›Paper›PMID 38566229›Full record

ArticleJournal of ovarian research2024

LncRNA SNHG12 promotes cell proliferation and inhibits apoptosis of granulosa cells in polycystic ovarian syndrome by sponging miR-129 and miR-125b.

Feilan Xuan, Ruiying Jin, Weimei Zhou, Yongju Ye, Yuefang Ren, Jiali Lu, Aixue Chen

Open access · goldAbstract read
In one paragraph

Article in Journal of ovarian research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Feilan XuanDepartment of Obstetrics and Gynecology, Hangzhou Traditional Chinese Medicine Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310007, China.
Ruiying JinDepartment of Gynecology, Jiaojiang Maternal and Child Health Hospital, Taizhou, Zhejiang, 318000, China.
Weimei ZhouDepartment of Ultrasound, Jiaojiang Maternal and Child Health Hospital, Taizhou, Zhejiang, 318000, China.
Yongju YeDepartment of Gynecology, Lishui Hospital of Traditional Chinese Medicine, Lishui, Zhejiang, 323000, China.
Yuefang RenDepartment of Gynecology, Huzhou Maternity & Child Health Care Hospital, Huzhou, Zhejiang, 313000, China.
Jiali LuDepartment of Gynecology, Huzhou Maternity & Child Health Care Hospital, Huzhou, Zhejiang, 313000, China.
Aixue ChenDepartment of Gynecology, Changxing People's Hospital of Chongming District, No.1008 Fengfu Road, Changxing Town, Chongming District, Shanghai, 201913, China. haiyangshatanbeike@126.com.
Huzhou Women and Children's Hospital · CNLishui Central Hospital · CNTaixing People's Hospital · CNZhejiang Chinese Medical University · CN

Funding

Zhejiang Medical and Health Science and Technology Plan 2022490202
6 · The paper itself

Abstract

backgroundPolycystic ovarian syndrome (PCOS) is the most common endocrine disease in women of childbearing age which is often associated with abnormal proliferation or apoptosis of granulosa cells (GCs). Studies proved that long non-coding RNA SNHG12 (lncRNA SNHG12) is significantly increased in ovarian cancer and cervical cancer patients and cells. The inhibition of lncRNA SNHG12 restrains the proliferation, migration, and invasion in tumor cells.

objectiveThis study explores the role of lncRNA SNHG12 in the apoptosis of GCs in PCOS and the underlying regulated mechanism.

methodsIn this study, the injection of dehydroepiandrosterone (DHEA) successfully induced the PCOS model in SD rats. The human granulosa-like tumor cell line KGN was incubated with insulin to assess the effects of lncRNA SNHG12 on GC proliferation and apoptosis.

resultsOverexpression of lncRNA SNHG12 influenced the body weight, ovary weight, gonadal hormone, and pathological changes, restrained the expressions of microRNA (miR)-129 and miR-125b, while downregulation of lncRNA SNHG12 exerted the opposite effects in PCOS rats. After silencing lncRNA SNHG12 in cells, the cell viability and proliferation were lessened whereas apoptosis of cells was increased. A loss-of-functions test was implemented by co-transfecting miR-129 and miR-125b inhibitors into lncRNA SNHG12-knocking down cells to analyze the effects on cell viability and apoptosis. Next, the existence of binding sites of SNHG12 and miR-129/miR-125b was proved based on the pull-down assay.

conclusionlncRNA SNHG12 might be a potential regulatory factor for the development of PCOS by sponging miR-129 and miR-125b in GCs.

Indexed as

MicroRNAsPolycystic Ovary SyndromeRNA, Long NoncodingAnimalsApoptosisCell ProliferationFemaleGranulosa CellsHumansRatsRats, Sprague-DawleyMicroRNAsMirn129 microRNA, humanRNA, Long NoncodingGranulosa cellsLncRNA SNHG12MiR-125bMiR-129Polycystic ovarian syndrome

Identifiers

PMID38566229
PMCPMC10986130
OpenAlexW4393397988

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.